Abstract

We propose to study the role of the cell surface in lymphoid cell differentiation by examining cell surface antigens determined by the Tla region of the mouse. This region controls a series of thymus-leukemia specific antigens called TL and a seris of lymphocyte antigens called Qa. Our approach will be to determine (1) how these cell surface antigens are controlled and expressed during lymphoid cell ontogeny and differentiation (2) how the Tla region genes regulate the expression of these cell surface components (3) what other cell surface antigens are controlled by the Tla region, and (4) what the relationship is between these Tla region antigens and those governed by the Major Histocompatibility Complex of the mouse. Our techniques will include immunization procedures, hybridoma production, serological definition of cell surface antigens by cytotoxicity testing, binding assays, and immunofluorescence, fluorescence-activated cell sorter analysis, and genetic linkage and complementation studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI012603-11
Application #
3125241
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1978-05-01
Project End
1986-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
New York State Department of Health
Department
Type
DUNS #
002436061
City
Menands
State
NY
Country
United States
Zip Code
12204

  Publications

Flaherty, L (1998) Generation, identification, and recovery of mouse mutations. Methods 14:107-18
Herron, B J; Silva, G H; Flaherty, L (1998) Putative assignment of ESTs to the genetic map by use of the SSLP database. Mamm Genome 9:1072-4
Baldocchi, R A; Flaherty, L (1997) Isolation of genomic fragments from polymorphic regions by representational difference analysis. Methods 13:337-46
Wang, Q; Flaherty, L (1996) Developmental expression of the mouse MHC Q genes. Eur J Immunogenet 23:121-7
Wang, Q; Geliebter, J; Tonkonogy, S et al. (1993) Expression of the Q2 gene of the MHC in thymus and intestinal epithelial cells. Immunogenetics 38:370-2
Wade, N; Flaherty, L (1993) DNA sequence analysis and polymorphism of the mouse CD4 gene. Immunogenetics 38:384
Waters, J B; Flaherty, L (1991) Expression and regulation of Q8b in a transfected cell line. Immunogenetics 34:179-84
Walsh, A C; Slack, J; Flaherty, L (1991) Characterization of several new MHC congenic strains. Immunogenetics 33:290-3
Tine, J A; Walsh, A; Rathbun, D et al. (1990) Genetic polymorphisms of Q region genes from wild-derived mice: implications for Q region evolution. Immunogenetics 31:315-25
Widacki, S M; Mehta, V; Flaherty, L et al. (1990) Biochemical differences in Qa-2 antigens expressed by Qa-2+,6+ and Qa-2+,6- strains. Evidence for differential expression of the Q7 and Q9 genes. Mol Immunol 27:559-70

Showing the most recent 10 out of 15 publications