Abstract

The dissemnation of plasmid DNA elements among bacteria provides one of the most important mechanisms through which bacteria gain new pathogenic traits and other genetic characteristics that complicate clinical therapy. Using the F plasmid conjugation system of Escherichia coli as a model, we are studying the genes and gene products required for the transmission of F DNA from one cell to another. During our genetic and protein product analysis of genes in the F transfer region we have discovered a number of previously unrecognized coding sequences, and identified activities that are involved in making F-pilin. We now plan to complete a DNA sequence analysis of the F DNA segment which includes transfer genes traC through trbB and to characterize the transfer related function of the new loci in this region. We also propose experiments designed to characterize the biochemical steps of F-pilin synthesis and the role of traQ and other tra functions in this process.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI014426-12
Application #
3125741
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-09-01
Project End
1993-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
12
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Texas A&M University
Department
Type
Schools of Medicine
DUNS #
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Maneewannakul, K; Kathir, P; Endley, S et al. (1996) Construction of derivatives of the F plasmid pOX-tra715: characterization of traY and traD mutants that can be complemented in trans. Mol Microbiol 22:197-205
Maneewannakul, K; Maneewannakul, S; Ippen-Ihler, K (1995) Characterization of traX, the F plasmid locus required for acetylation of F-pilin subunits. J Bacteriol 177:2957-64
Maneewannakul, S; Maneewannakul, K; Ippen-Ihler, K (1994) The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31:300-7
Moore, D; Hamilton, C M; Maneewannakul, K et al. (1993) The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin. J Bacteriol 175:1375-83
Maneewannakul, K; Maneewannakul, S; Ippen-Ihler, K (1993) Synthesis of F pilin. J Bacteriol 175:1384-91
Maneewannakul, K; Ippen-Ihler, K (1993) Construction and analysis of F plasmid traR, trbJ, and trbH mutants. J Bacteriol 175:1528-31
Maneewannakul, S; Maneewannakul, K; Ippen-Ihler, K (1992) Characterization, localization, and sequence of F transfer region products: the pilus assembly gene product TraW and a new product, TrbI. J Bacteriol 174:5567-74
Maneewannakul, S; Kathir, P; Ippen-Ihler, K (1992) Characterization of the F plasmid mating aggregation gene traN and of a new F transfer region locus trbE. J Mol Biol 225:299-311
Maneewannakul, K; Maneewannakul, S; Ippen-Ihler, K (1992) Sequence alterations affecting F plasmid transfer gene expression: a conjugation system dependent on transcription by the RNA polymerase of phage T7. Mol Microbiol 6:2961-73
Maneewannakul, S; Maneewannakul, K; Ippen-Ihler, K (1991) Characterization of trbC, a new F plasmid tra operon gene that is essential to conjugative transfer. J Bacteriol 173:3872-8

Showing the most recent 10 out of 21 publications