Abstract

During the present grant period we propose to carry out studies in 3 areas relating to proteins expressed on the surface of human T cells: 1) Human T Cell Receptors. The expression of both TCR alpha beta and TCR gamma delta will be studied using cloned genes encoding the various chains of these receptors. Some aspects of the cell biology and functions of these receptors will be examined. The ultimate goal is to engineer these genes in order to produce soluble TCR alpha beta and TCR gamma delta, to produce these soluble receptors in large amounts and to crystallize them. 2) Pulmonary sarcoidosis. A primary goal is to culture and clone the T cells from bronchoalveolar lavage of patients with this disease with a view to characterizing them phenotypically and functionally. In particular we wish to examine the question of whether or not these T cells are oligoclonal and utilize one or a small number of T cell receptor alpha and beta gene V, D and J segments. 3) The 4F2 protein. This heterodimeric protein is the first activation antigen detected on the surface of human T cells. We wish to complete the cloning of the genes of this protein and use them to study the mechanism of activation of their transcription and their role in cell proliferation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015669-14
Application #
3126344
Study Section
Experimental Immunology Study Section (EI)
Project Start
1978-08-01
Project End
1993-07-31
Budget Start
1991-08-01
Budget End
1992-07-31
Support Year
14
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Pasqualini, R; Bodorova, J; Ye, S et al. (1993) A study of the structure, function and distribution of beta 5 integrins using novel anti-beta 5 monoclonal antibodies. J Cell Sci 105 ( Pt 1):101-11
Sleckman, B P; Shin, J; Igras, V E et al. (1992) Disruption of the CD4-p56lck complex is required for rapid internalization of CD4. Proc Natl Acad Sci U S A 89:7566-70
Maziarz, R T; Groh, V; Prendergast, M et al. (1991) Non-MHC-restricted target-cell lysis by a CD4-CD8- TCR alpha beta T-cell line, as well as by TCR gamma delta T-cell lines, results from lymphokine-activated killing. Int J Cancer 48:142-7
Doyle, C; Ford, P J; Ponath, P D et al. (1990) Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes. Proc Natl Acad Sci U S A 87:4590-4
Strominger, J L; Santos-Aguado, J; Burakoff, S J et al. (1989) Mutants of HLA-A2 in the analysis of its structure and function. Cold Spring Harb Symp Quant Biol 54 Pt 1:361-3
Band, H; Hochstenbach, F; Parker, C M et al. (1989) Expression of human T cell receptor-gamma delta structural forms. J Immunol 142:3627-33
Santos-Aguado, J; Crimmins, M A; Mentzer, S J et al. (1989) Alloreactivity studied with mutants of HLA-A2. Proc Natl Acad Sci U S A 86:8936-40
Groh, V; Porcelli, S; Fabbi, M et al. (1989) Human lymphocytes bearing T cell receptor gamma/delta are phenotypically diverse and evenly distributed throughout the lymphoid system. J Exp Med 169:1277-94
Nishimura, Y; Bierer, B E; Jones, W K et al. (1988) Expression and function of a CD5 cDNA in human and murine T cells. Eur J Immunol 18:747-53
Leiden, J M; Gottesdiener, K M; Quertermous, T et al. (1988) T-cell receptor gene rearrangement and expression in human natural killer cells: natural killer activity is not dependent on the rearrangement and expression of T-cell receptor alpha, beta, or gamma genes. Immunogenetics 27:231-8

Showing the most recent 10 out of 34 publications