The overall objective of this proposal is to investigate the natural history of sexually acquired cytomegalovirus (CMV) infection. Using both prevalence surveys and prospective cohort studies we will systematically analyze and correlate CMV infection with sexual practices, level of sexual activity, changes in sex partners and other correlates of sexual activity. Cohort studies to assess the frequency of acquisition of disease, anatomic sites and duration of excretion of CMV from genitourinary sites in clients of sexually transmitted disease clinics, university health center populations, and adolescents attending medical clinics will be performed. Sex partners of persons with and without CMV infection will be followed to evaluate the frequency of CMV transmission from contact with persons with persistent or chronic CMV infection. Restriction enzyme typing will be utilized to compare isolates from sex partners in order to distinguish endogenous relapse with exogenous reinfection. Both local and systemic host immune responses to specific viral polypeptides using radioimmunoprecipitation and Western blot analysis will be performed. In situ hybridization techniques for the detection of CMV DNA will be performed in order to assess the sites of CMV in the genitourinary tract. Comparison of conventional isolation methods, detection of CMV antigen with monoclonal antibodies and CMV DNA by Southern blotting and in situ hybridization methods with clinical expression and transmission of disease will further our understanding of the natural history and persistence of CMV infection of the genitourinary tract.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018649-05
Application #
3128091
Study Section
Epidemiology and Disease Control Subcommittee 3 (EDC)
Project Start
1982-01-01
Project End
1987-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Coonrod, D; Collier, A C; Ashley, R et al. (1998) Association between cytomegalovirus seroconversion and upper genital tract infection among women attending a sexually transmitted disease clinic: a prospective study. J Infect Dis 177:1188-93
Collier, A C; Handsfield, H H; Ashley, R et al. (1995) Cervical but not urinary excretion of cytomegalovirus is related to sexual activity and contraceptive practices in sexually active women. J Infect Dis 171:33-8
Collier, A C; Handsfield, H H; Roberts, P L et al. (1990) Cytomegalovirus infection in women attending a sexually transmitted disease clinic. J Infect Dis 162:46-51
Collier, A C; Chandler, S H; Handsfield, H H et al. (1989) Identification of multiple strains of cytomegalovirus in homosexual men. J Infect Dis 159:123-6
Gold, D; Ashley, R; Abbo, H et al. (1988) Analysis of a major target of the human immune response to cytomegalovirus using monoclonal antibodies. J Med Virol 25:237-43
Ochs, H D; Junker, A K; Collier, A C et al. (1988) Abnormal antibody responses in patients with persistent generalized lymphadenopathy. J Clin Immunol 8:57-63
Gold, D; Ashley, R; Handsfield, H H et al. (1988) Immunoblot analysis of the humoral immune response in primary cytomegalovirus infection. J Infect Dis 157:319-26
Collier, A C; Meyers, J D; Murphy, V L et al. (1987) Relationship between antibody to LAV/HTLV-III and the natural course of subclinical cellular immune dysfunction in homosexual men. Sex Transm Dis 14:1-8
Daling, J R; Weiss, N S; Hislop, T G et al. (1987) Sexual practices, sexually transmitted diseases, and the incidence of anal cancer. N Engl J Med 317:973-7
Chandler, S H; Handsfield, H H; McDougall, J K (1987) Isolation of multiple strains of cytomegalovirus from women attending a clinic for sexually transmitted disease. J Infect Dis 155:655-60

Showing the most recent 10 out of 16 publications