Abstract

The objective of these studies is to analyze the fine structure of the antibody combining sites which bind a defined, site filling antigenic determinant of six or seven Alpha(1 Yield 6)-linked glucose residues. Changes in the amino acid sequence of the variable regions of heavy and light chains of mouse hybridoma and myeloma anti-Alpha(1 Yield 6) dextrans will be correlated with changes in combining site size, affinity and idiotypic determinants. The relative contributions of CDR1, 2, and 3 and of VH and VL to the combining site will be assessed. The origins of antibody diversity (e.g., somatic or germ-line) to this well defined, T independent antigen will be determined. Chain recombination and in vitro mutagenesis with expression of the mutagenized chains by gene transfection will be used to further define the contribution of specific amino acid residues to the combining specificity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI019042-04
Application #
3128465
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1983-09-30
Project End
1989-08-31
Budget Start
1986-09-30
Budget End
1987-08-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027

  Publications

Wu, A M; Wu, J H; Chen, Y Y et al. (1999) Further characterization of the combining sites of Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A(4). Glycobiology 9:1161-70
Wu, J H; Song, S C; Chen, Y Y et al. (1998) Multi-antennary Gal beta1-->4GlcNAc and Gal beta1-->3GalNAc clusters as important ligands for a lectin isolated from the sponge Geodia cydonium. FEBS Lett 427:134-8
Wu, A M; Song, S C; Chang, S C et al. (1997) Further characterization of the binding properties of a GalNAc specific lectin from Codium fragile subspecies tomentosoides. Glycobiology 7:1061-6
Wu, A M; Wu, J H; Song, S C et al. (1996) Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4, reacting with Tn (Ga1NAc alpha1 --> Ser/Thr) or galabiose (Ga1 alpha1 --> 4Ga1) containing ligands. FEBS Lett 398:183-6
Nickerson, K G; Tao, M H; Chen, H T et al. (1995) Human and mouse monoclonal antibodies to blood group A substance, which are nearly identical immunochemically, use radically different primary sequences. J Biol Chem 270:12457-65
Wu, A M; Song, S C; Wu, J H et al. (1995) Affinity of Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin B4 for Gal alpha 1-->4 Gal ligand. Biochem Biophys Res Commun 216:814-20
Liao, J; Nickerson, K G; Bystricky, S et al. (1995) Characterization of a human monoclonal immunoglobulin M (IgM) antibody (IgMBEN) specific for Vi capsular polysaccharide of Salmonella typhi. Infect Immun 63:4429-32
Chen, J; Borden, P; Liao, J et al. (1992) Variable region cDNA sequences of three mouse monoclonal anti-idiotypic antibodies specific for anti-alpha(1----6)dextrans with groove- or cavity-type combining sites. Mol Immunol 29:1121-9
Schiffer, M; Kabat, E A; Wu, T T (1992) Subgroups of Tcr alpha chains and correlation with T-cell function. Immunogenetics 35:224-34
Padlan, E A; Kabat, E A (1991) Modeling of antibody combining sites. Methods Enzymol 203:3-21

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