Abstract

We will investigate the replication mechanisms of the maxicircle component of the kinetoplast DNA (kDNA) of the trypanosome Crithidia fasciculata. Autonomous replication sequences (ARS) present in maxicircle DNA will be selected by cloning into yeast plasmids carrying a selectable yeast gene but lacking the capability for autonomous replication. Cloned ARSs will be sub-cloned to identify the minimal functional sequence and to allow rapid DNA sequence analysis. Plasmids carrying a selectable drug-resistance gene will be constructed to allow the development of DNA transformation techniques for trypanosomes and to provide appropriate vectors for cloning genes involved in the replication of kDNA. Cloned ARSs will also be used to identify proteins that interact specifically with maxicircle ARSs and as templates for studies of the in vitro replication of maxicircle DNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020080-03
Application #
3129604
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1983-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095