The synthesis, processing and assembly of viral RNA and proteins will be studied in vivo and in vitro in order to (i) discover the pathway of picornavirus morphogenesis, and (ii) duplicate this pathway in vitro. In addition, a messenger RNA (mRNA)-dependent rabbit reticulocyte lysate (MDL) programmed with purified poliovirus RNA will be used so that incubation conditions can be devised to promote the synthesis and assembly of viral morphogenetic intermediates de novo from polypeptides synthesized in vitro. The achievement of these goals will lead to an understanding of the morphogenesis of picornaviruses in particular and that of other non-enveloped viruses in general. The capability of synthesizing functional viral morphogenetic intermediates (i.e., viral capsids) in vitro may eventually permit: (i) the selective encapsidation of modified viral or cellular nucleic acids for delivery to specific cells possessing receptors for such capsids, and (ii) the morphogenetic engineering of avirulent or attenuated virus strains for disease prophylaxis.
| Onodera, S; Phillips, B A (1987) A novel method for obtaining poliovirus 14 S pentamers from procapsids and their self-assembly into virus-like shells. Virology 159:278-87 |
| Onodera, S; Cardamone Jr, J J; Phillips, B A (1986) Biological activity and electron microscopy of poliovirus 14S particles obtained from alkali-dissociated procapsids. J Virol 58:610-8 |
| Naclerio, R M; Proud, D; Togias, A G et al. (1985) Inflammatory mediators in late antigen-induced rhinitis. N Engl J Med 313:65-70 |
| Togias, A; Naclerio, R M; Proud, D et al. (1985) Mediator release during nasal provocation. A model to investigate the pathophysiology of rhinitis. Am J Med 79:26-33 |
