Abstract

We will study the function and genetics of the Ia and H-2 K/D antigens of the mouse major histocompatibility complex. These will be accomplished by production of a panel of monoclonals all directed at a single H-2 haplotype, combined with production of panels of helper and cytotoxic clones also recognizing the same H-2 molecules. These approaches will allow us to define the portions of Ia and K/D molecules used for recognition by T cells. In addition, we will define restriction fragment length polymorphisms on Chromosome 17 in order to provide both additional genetic markers and reveal the number and position of DNA sequences which are H-2 related. Finally we will determine precisely the role of K/D molecules and their domain by combining the CTL clones and DNA mediated gene transfer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020288-04
Application #
3129857
Study Section
Immunobiology Study Section (IMB)
Project Start
1983-02-01
Project End
1988-01-31
Budget Start
1986-02-01
Budget End
1987-01-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Wang, Bo; Sharma, Ashawni; Maile, Robert et al. (2002) Peptidic termini play a significant role in TCR recognition. J Immunol 169:3137-45
Wang, B; Norbury, C C; Greenwood, R et al. (2001) Multiple paths for activation of naive CD8+ T cells: CD4-independent help. J Immunol 167:1283-9
Abdel-Motal, U M; Friedline, R; Poligone, B et al. (2001) Dendritic cell vaccination induces cross-reactive cytotoxic T lymphocytes specific for wild-type and natural variant human immunodeficiency virus type 1 epitopes in HLA-A*0201/Kb transgenic mice. Clin Immunol 101:51-8
Cairns, B A; Maile, R; Buchanan, I et al. (2001) CD8(+) T cells express a T-helper 1--like phenotype after burn injury. Surgery 130:210-6
Maile, R; Wang, B; Schooler, W et al. (2001) Antigen-specific modulation of an immune response by in vivo administration of soluble MHC class I tetramers. J Immunol 167:3708-14
Wang, B; Maile, R; Greenwood, R et al. (2000) Naive CD8+ T cells do not require costimulation for proliferation and differentiation into cytotoxic effector cells. J Immunol 164:1216-22
Wu, Y P; McMahon, E; Kraine, M R et al. (2000) Distribution and characterization of GFP(+) donor hematogenous cells in Twitcher mice after bone marrow transplantation. Am J Pathol 156:1849-54
Serody, J S; Collins, E J; Tisch, R M et al. (2000) T cell activity after dendritic cell vaccination is dependent on both the type of antigen and the mode of delivery. J Immunol 164:4961-7
Mylin, L M; Schell, T D; Roberts, D et al. (2000) Quantitation of CD8(+) T-lymphocyte responses to multiple epitopes from simian virus 40 (SV40) large T antigen in C57BL/6 mice immunized with SV40, SV40 T-antigen-transformed cells, or vaccinia virus recombinants expressing full-length T antigen or epitop J Virol 74:6922-34
Batalia, M A; Kirksey, T J; Sharma, A et al. (2000) Class I MHC is stabilized against thermal denaturation by physiological concentrations of NaCl. Biochemistry 39:9030-8

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