Abstract

This grant application involves a study of the relationships between common airborne mold spores and bronchial asthma in man. The objectives are to identify, isolate and characterize clinically relevant allergens from common airborne mold spores through development of in vivo and in vitro biological assays for these allergens. The type of respiratory symptoms in patients with mold induced respiratory disease will be assessed by questionnaire and response to bronchoprovocation challenge testing. Once clinically relevant molds have been identified, in vitro assays will be developed to detect allergens. Clinically relevant mold allergens will be isolated and characterized utilizing various extraction and analytical techniques including CIE, CRIE, HPLC, polyacrylamide gel electrophoresis and RAST inhibition. Such studies will help identify clinically relevant fungal species that induce asthma in man. They will also provide valuable information concerning the biologic properties and potency of fungal allergenic extracts used for skin testing and immunotherapy. In addition, they will allow us to identify which in vitro assays are helpful for standardization of mold allergens and identification of mold sensitive patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020331-03
Application #
3129934
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1983-09-01
Project End
1987-03-31
Budget Start
1985-09-01
Budget End
1987-03-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Tulane University
Department
Type
Schools of Medicine
DUNS #
City
New Orleans
State
LA
Country
United States
Zip Code
70118

  Publications

Lehrer, S B; Hughes, J M; Altman, L C et al. (1994) Prevalence of basidiomycete allergy in the USA and Europe and its relationship to allergic respiratory symptoms. Allergy 49:460-5
Lehrer, S B (1994) Compliance and acceptance problems with standardized extracts. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M :211-7
Wongtim, S; Lehrer, S B; Salvaggio, J E et al. (1993) Protease activity in cockroach and basidiomycete allergen extracts. Allergy Proc 14:263-8
Horner, W E; Helbling, A; Lehrer, S B (1993) Basidiomycete allergens: comparison of three Ganoderma species. Allergy 48:110-6
Helbling, A; Horner, W E; Lehrer, S B (1993) Comparison of Psilocybe cubensis spore and mycelium allergens. J Allergy Clin Immunol 91:1059-66
Helbling, A; Horner, W E; Lehrer, S B (1993) Identification of Psilocybe cubensis spore allergens by immunoprinting. Int Arch Allergy Immunol 100:263-7
Horner, W E; Levetin, E; Lehrer, S B (1993) Basidiospore allergen release: elution from intact spores. J Allergy Clin Immunol 92:306-12
Horner, W E; Lopez, M; Salvaggio, J E et al. (1991) Basidiomycete allergy: identification and characterization of an important allergen from Calvatia cyathiformis. Int Arch Allergy Appl Immunol 94:359-61
Sastre, J; Ibanez, M D; Lopez, M et al. (1990) Respiratory and immunological reactions among Shiitake (Lentinus edodes) mushroom workers. Clin Exp Allergy 20:13-9
Lehrer, S B; Salvaggio, J E (1990) Allergens: standardization and impact of biotechnology--a review. Allergy Proc 11:197-208

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