Abstract

The proposed project is intended to provide a better understanding of the physiology and metabolism of L. pneumophila through determination of essential nutrient requirements, incorporation of radiolabeled compounds, oxygen uptake in the presence of specific substrates, cellular enzyme activities, and capabilities to survive periods of nutrient deprivation. Analysis of the nutrient requirements in a defined medium have shown that arginine, cysteine, isoleucine, leucine, methionine, serine, threonine, and valine were required for growth. A medium containing just these eight amino acids supported the growth of L. pneumophila only if further supplemented with glutamic acid. The inorganic ions magnesium, phosphate, and potassium were also required for growth in defined medium. Oxygen uptake studies indicated that serine, oxaloacetate, pyruvate, glutamate, tyrosine and threonine stimulated respiration of washed cells of this organism. Extracellular enzymes produced by all strains of L. pneumophila include protease, lipase, acid phosphatase, nuclease(s), alpha-amylase and beta-lactamase. No elastase, collagenase, phospholipase C, hyaluronidase, chondroitinase, neuraminidase or coagulase was detected. Starvation survival studies indicated that the greatest survival was achieved with a high cell density, at 25 degrees (or less) in a buffered solution at pH6.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI023567-02
Application #
3135867
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1985-09-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Louisville
Department
Type
School of Medicine & Dentistry
DUNS #
City
Louisville
State
KY
Country
United States
Zip Code
40292

  Publications

Sahney, N N; Summersgill, J T; Ramirez, J A et al. (2001) Inhibition of oxidative burst and chemotaxis in human phagocytes by Legionella pneumophila zinc metalloprotease. J Med Microbiol 50:517-25
Liu, K; Moliterno, R A; Fu, X F et al. (1997) Identification of two types of autoreactive T lymphocyte clones cultured from cardiac allograft-infiltrating cells incubated with recombinant mycobacterial heat shock protein 71. Transpl Immunol 5:57-65
Liu, K; Moliterno, R A; Qian, J et al. (1996) Role of heat shock proteins in heart transplant rejection. J Heart Lung Transplant 15:222-8
Qian, J; Moliterno, R; Donovan-Peluso, M A et al. (1995) Expression of stress proteins and lymphocyte reactivity in heterotopic cardiac allografts undergoing cellular rejection. Transpl Immunol 3:114-23
Moliterno, R; Valdivia, L; Pan, F et al. (1995) Heat shock protein reactivity of lymphocytes isolated from heterotopic rat cardiac allografts. Transplantation 59:598-604
Duquesnoy, R J; Moliterno, R; Qian, J et al. (1995) Role of heat shock protein immunity in allograft rejection. Transplant Proc 27:468-70
Woan, M C; Moliterno, R M; Zeevi, A et al. (1993) Characterization of the biopsy growth assay in monitoring cardiac transplant patients for rejection. Transplant Proc 25:946-7
Sahney, N N; Lambe, B C; Summersgill, J T et al. (1990) Inhibition of polymorphonuclear leukocyte function by Legionella pneumophila exoproducts. Microb Pathog 9:117-25
Summersgill, J T; Raff, M J; Miller, R D (1990) Interactions of virulent and avirulent Legionella pneumophila with human monocytes. J Leukoc Biol 47:31-8
Bortner, C A; Arnold, R R; Miller, R D (1989) Bactericidal effect of lactoferrin on Legionella pneumophila: effect of the physiological state of the organism. Can J Microbiol 35:1048-51

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