Abstract

. The following experimental studies are proposed in this application: 1. Study the molecular biology of mycoplasma fermentans (the incognitus strain) which has direct implication in clinical diagnosis, in understanding pathogenesis, and in future vaccine development. 2. To identify M. fermentans specific repetitive DNA sequences to be used to develop highly sensitive and specific PCR assays to detect M. fermentans related mycoplasma infection in patients with AIDS. 3. To develop serological assays detecting specific antibodies produced in patients with AIDS against surface antigens of M. fermentans. 4. To develop reliable and practical culture systems, to identify the best disease stage for direct isolation, and to characterize the new clinical isolates of M. fermentans related mycoplasmas from AIDS patients. 5. To correlate the results of antibody positivity, PCR evidence of the mycoplasma infection and culture isolation with disease stages of AIDS. To assess if the presence of mycoplasma infection plays any role in the disease of AIDS.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI031830-02
Application #
3146840
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Project Start
1991-09-01
Project End
1994-07-31
Budget Start
1992-08-01
Budget End
1993-07-31
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
American Registry of Pathology, Inc.
Department
Type
DUNS #
114400633
City
Washington
State
DC
Country
United States
Zip Code
20306

  Publications

Hu, W S; Hayes, M M; Wang, R Y et al. (1998) High-frequency DNA rearrangements in the chromosomes of clinically isolated Mycoplasma fermentans. Curr Microbiol 37:1-5
Zhang, B; Shih, J W; Wear, D J et al. (1997) High-level expression of H-ras and c-myc oncogenes in mycoplasma-mediated malignant cell transformation. Proc Soc Exp Biol Med 214:359-66
Hayes, M M; Li, B J; Wear, D J et al. (1996) Pathogenicity of Mycoplasma fermentans and Mycoplasma penetrans in experimentally infected chicken embryos. Infect Immun 64:3419-24
Tsai, S; Wear, D J; Shih, J W et al. (1995) Mycoplasmas and oncogenesis: persistent infection and multistage malignant transformation. Proc Natl Acad Sci U S A 92:10197-201
Hayes, M M; Foo, H H; Timenetsky, J et al. (1995) In vitro antibiotic susceptibility testing of clinical isolates of Mycoplasma penetrans from patients with AIDS. Antimicrob Agents Chemother 39:1386-7
Feng, S H; Lo, S C (1994) Induced mouse spleen B-cell proliferation and secretion of immunoglobulin by lipid-associated membrane proteins of Mycoplasma fermentans incognitus and Mycoplasma penetrans. Infect Immun 62:3916-21
Hu, W S; Wang, R Y; Shih, J W et al. (1993) Identification of a putative infC-rpmI-rplT operon flanked by long inverted repeats in Mycoplasma fermentans (incognitus strain). Gene 127:79-85
Lo, S C; Hayes, M M; Kotani, H et al. (1993) Adhesion onto and invasion into mammalian cells by mycoplasma penetrans: a newly isolated mycoplasma from patients with AIDS. Mod Pathol 6:276-80
Wise, K S; Kim, M F; Theiss, P M et al. (1993) A family of strain-variant surface lipoproteins of Mycoplasma fermentans. Infect Immun 61:3327-33
Dawson, M S; Hayes, M M; Wang, R Y et al. (1993) Detection and isolation of Mycoplasma fermentans from urine of human immunodeficiency virus type 1-infected patients. Arch Pathol Lab Med 117:511-4

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