Abstract

Dr. Ganz proposes to test the hypothesis that transgenic expression of antimicrobial peptides targeted to neutrophil granules will enhance the resistance of recipient mice to microbial pathogens. The following aims are proposed: 1) Optimize the expression of defensin and protegrin genes in the mouse cell line 32D c13; 2) Construct transgenic mice that express human defensin; 3) Assay the effects of transgenic peptides on the antimicrobial activity of mouse phagocytes and body fluids against a panel of pathogens; 4) Determine the effect of transgenic antimicrobial peptides on the susceptibility of mice to selected pathogens.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI038567-03
Application #
2457842
Study Section
Special Emphasis Panel (ZRG5-BM-2 (05))
Project Start
1995-09-30
Project End
1998-07-31
Budget Start
1997-08-01
Budget End
1998-07-31
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Linzmeier, R; Ho, C H; Hoang, B V et al. (1999) A 450-kb contig of defensin genes on human chromosome 8p23. Gene 233:205-11
Liu, L; Wang, L; Jia, H P et al. (1998) Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation. Gene 222:237-44