Abstract

To expedite the identification of potential Cryptosporidium chemotherapeutic and immunotherapeutic targets and to provide nucleic acid sequence information that will allow immediate isolation of the corresponding genes a focused, short-term project whose goal is the determination and rapid public distribution of 5,000 C. parvum gene sequence tags (GST's 300-600 bp of single-pass nucleotide sequence from random genomic DNA clones) is proposed.
The specific aims of the application are: (1) Construct and characterize randomly-sheared genomic DNA libraries from the Iowa isolate of C. parvum, (2) Determine 5,000 C. parvum GST's by single-pass automated fluorescent sequencing of both ends of 2,500 random genomic DNA clones, and (3) Deposit GST sequences as they are completed in the public GenBank genome survey sequence database (dbGSS) and establish an Internet site devoted to distribution and analyses of the C. parvum GST's determined in this investigation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI042565-01
Application #
2545299
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Project Start
1997-12-15
Project End
1999-11-30
Budget Start
1997-12-15
Budget End
1998-11-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143