Abstract

This project describes a multidisciplinary effort to evaluate the feasibility of using transgenic plants as a source for an oral Hepatitis B vaccine. There are two elements of the research plan. First, quantitative immune response to oral presentation of recombinant HBsAg (as isolated spherical particles or in plant tissues) will be evaluated with Special emphasis on systemic immunity in relation to levels, of HBsAg dosage. Secondly the cellular mechanisms regulating, HBsAg spherical particle assembly plant cells, will continue to be systemically evaluated and compared to, information of analogous processes in mammalian cells to achieve enhanced HBsAg accumulation in transgenic plants. Preliminary data provides indications for the success of the proposed research. Evidence for both priming and boosting of systemic immune responses in mice b) oral immunization with either isolated HBsAg particles or with transgenic potatoes expressing HBsAg is provided. Additionally, a mucosal anti- HBs response might create an improved and more cost effective vaccination strategy for HBV immunization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI042836-03
Application #
6170769
Study Section
Virology Study Section (VR)
Program Officer
Johnson, Leslye D
Project Start
1998-09-30
Project End
2002-11-30
Budget Start
2000-09-01
Budget End
2002-11-30
Support Year
3
Fiscal Year
2000
Total Cost
$300,015
Indirect Cost

  Institution

Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263

  Publications

Lugade, Amit A; Kalathil, Suresh; Heald, Jonathan L et al. (2010) Transgenic plant-based oral vaccines. Immunol Invest 39:468-82
Thanavala, Yasmin; Lugade, Amit A (2010) Oral transgenic plant-based vaccine for hepatitis B. Immunol Res 46:4-11
Huang, Zhong; LePore, Kate; Elkin, Galina et al. (2008) High-yield rapid production of hepatitis B surface antigen in plant leaf by a viral expression system. Plant Biotechnol J 6:202-9
Mayo, Kristin J; Gonzales, Barbara J; Mason, Hugh S (2006) Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens. Nat Protoc 1:1105-11
Huang, Zhong; Santi, Luca; LePore, Kate et al. (2006) Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice. Vaccine 24:2506-13
Thanavala, Yasmin; Mahoney, Martin; Pal, Sribani et al. (2005) Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci U S A 102:3378-82
Huang, Zhong; Elkin, Galina; Maloney, Bryan J et al. (2005) Virus-like particle expression and assembly in plants: hepatitis B and Norwalk viruses. Vaccine 23:1851-8
Sojikul, Punchapat; Buehner, Norene; Mason, Hugh S (2003) A plant signal peptide-hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells. Proc Natl Acad Sci U S A 100:2209-14
Smith, Mark L; Richter, Lizabeth; Arntzen, Charles J et al. (2003) Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies. Vaccine 21:4011-21
Kong, Q; Richter, L; Yang, Y F et al. (2001) Oral immunization with hepatitis B surface antigen expressed in transgenic plants. Proc Natl Acad Sci U S A 98:11539-44

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