Abstract

Individuals infected with HIV-1 often make vigorous anti-viral CD8 cytotoxic T lymphocyte (CTL) responses. The CTL appear to play an important role in reducing the viral load but infection usually persists and eventually worsens. The long-term goal of this project to is facilitate the development of vaccines aimed at generating more effective CD8 CTL responses than occur in the course of natural infection. To this end we will define the maximally effective forms of heat shock fusion protein and DNA vectors as immunogens for eliciting potent primary and memory CD8 cytotoxic T lymphocyte (CTL) responses in a transgenic mouse model with aim of eventually applying related immunogens to the vaccination of nonhuman primates (macaque monkeys?) against SIV infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI044477-05
Application #
6511093
Study Section
Special Emphasis Panel (ZAI1-VSG-A)
Program Officer
Bridges, Sandra H
Project Start
1998-05-01
Project End
2004-04-30
Budget Start
2002-05-01
Budget End
2004-04-30
Support Year
5
Fiscal Year
2002
Total Cost
$316,395
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Internal Medicine/Medicine
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Palliser, Deborah; Guillen, Eduardo; Ju, Mindy et al. (2005) Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells. J Immunol 174:1879-87
Palliser, Deborah; Huang, Qian; Hacohen, Nir et al. (2004) A role for Toll-like receptor 4 in dendritic cell activation and cytolytic CD8+ T cell differentiation in response to a recombinant heat shock fusion protein. J Immunol 172:2885-93
Rudolph, Markus G; Shen, Lucy Q; Lamontagne, Stephen A et al. (2004) A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects. J Immunol 172:2994-3002
Ge, Qing; Eisen, Herman N; Chen, Jianzhu (2004) Use of siRNAs to prevent and treat influenza virus infection. Virus Res 102:37-42
Chen, Jianzhu; Eisen, Herman N; Kranz, David M (2003) A model T-cell receptor system for studying memory T-cell development. Microbes Infect 5:233-40
Ge, Qing; Bai, Ailin; Shen, Ching-Hung et al. (2003) CD4+ T-cell responses to self-peptide--MHC. Trends Immunol 24:186-9
Ge, Qing; McManus, Michael T; Nguyen, Tam et al. (2003) RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc Natl Acad Sci U S A 100:2718-23
Ge, Qing; Palliser, Deborah; Eisen, Herman N et al. (2002) Homeostatic T cell proliferation in a T cell-dendritic cell coculture system. Proc Natl Acad Sci U S A 99:2983-8
Ge, Qing; Hu, Hui; Eisen, Herman N et al. (2002) Naive to memory T-cell differentiation during homeostasis-driven proliferation. Microbes Infect 4:555-8
Sugawa, Satoshi; Palliser, Deborah; Eisen, Herman N et al. (2002) How do cultured CD8(+) murine T cell clones survive repeated ligation of the TCR? Int Immunol 14:23-30

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