Abstract

This grant is targeted towards the PI's long-term focus to characterize the structure and function of the midgut of mosquitoes. This grant focuses upon elucidating the molecular processes involved in nutrient uptake in Aedes aegypti. Through a molecular approach to identify transport processes at the midgut, the PI has isolated cDNA's for 3 amino-acid transporters, a potential protein needed to form a functional heterodimer with the amino acid transporters, and a putative sucrose transporter from the midgut of Aedes aegypti. In objective 1, the PI proposes to continue in the isolation of full-length cDNA clones for two of the proteins for which he has partial cDNAs. These cDNAs will be expressed in a heterologous expression system (Xenopus oocytes) and this system used to characterize the substrate selectivity of each of the amino acid transporters and the ion selectivity of the transport process. In the second objective, similar experiments will be performed for the sucrose transporter. As the final objective, the in vivo regulation of these transporters will be analyzed. The expression of the genes will be analyzed in vivo to determine if there is developmental regulation and modulation following either sugar or blood feeding.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI048049-05
Application #
6752733
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Costero, Adriana
Project Start
2000-09-01
Project End
2006-05-31
Budget Start
2004-06-01
Budget End
2006-05-31
Support Year
5
Fiscal Year
2004
Total Cost
$258,125
Indirect Cost

  Institution

Name
University of California Riverside
Department
Anatomy/Cell Biology
Type
Schools of Earth Sciences/Natur
DUNS #
627797426
City
Riverside
State
CA
Country
United States
Zip Code
92521

  Publications

Evans, Amy M; Aimanova, Karlygash G; Gill, Sarjeet S (2009) Characterization of a blood-meal-responsive proton-dependent amino acid transporter in the disease vector, Aedes aegypti. J Exp Biol 212:3263-71
Pullikuth, Ashok K; Aimanova, Karlygash; Kang'ethe, Wanyoike et al. (2006) Molecular characterization of sodium/proton exchanger 3 (NHE3) from the yellow fever vector, Aedes aegypti. J Exp Biol 209:3529-44
Patrick, Marjorie L; Aimanova, Karlygash; Sanders, Heather R et al. (2006) P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti. J Exp Biol 209:4638-51
Sanders, Heather R; Foy, Brian D; Evans, Amy M et al. (2005) Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti. Insect Biochem Mol Biol 35:1293-307
Umesh, Anita; Cohen, Bruce N; Ross, Linda S et al. (2003) Functional characterization of a glutamate/aspartate transporter from the mosquito Aedes aegypti. J Exp Biol 206:2241-55
Sanders, Heather R; Evans, Amy M; Ross, Linda S et al. (2003) Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti. Insect Biochem Mol Biol 33:1105-22
Jin, Xiangyang; Aimanova, Karlygash; Ross, Linda S et al. (2003) Identification, functional characterization and expression of a LAT type amino acid transporter from the mosquito Aedes aegypti. Insect Biochem Mol Biol 33:815-27
Pullikuth, Ashok K; Filippov, Valeri; Gill, Sarjeet S (2003) Phylogeny and cloning of ion transporters in mosquitoes. J Exp Biol 206:3857-68
Sandhu, Sumandeep K; Ross, Linda S; Gill, Sarjeet S (2002) A cocaine insensitive chimeric insect serotonin transporter reveals domains critical for cocaine interaction. Eur J Biochem 269:3934-44
Pullikuth, Ashok K; Gill, Sarjeet S (2002) In vivo membrane trafficking role for an insect N-ethylmaleimide-sensitive factor which is developmentally regulated in endocrine cells. J Exp Biol 205:911-26

Showing the most recent 10 out of 13 publications