The objectives of this program are to establish reagents and methods for rapid, simultaneous molecular detection of a wide range of viruses. Oligonucleotide primer sets useful for detection of some viral sequences by consensus PCR (cPCR) are established; however, at present there are neither comprehensive databases wherein investigators can obtain core sequences conserved within viral families, nor algorithms suitable for extracting such core sequences. We will create algorithms for extraction of core sequences; build virtual libraries of core sequences; synthesize primers suitable for detection of core sequences; and clone core sequences from representative viral families. These tools will be used to develop three complementary technologies for pathogen identification: cDNA microarrays, Domain-Specific Differential Display (DSDD), and multiplexed bead-based flow cytometric (MB-BFC) assays. Assays will be optimized using synthetic viral nucleic acid targets, validated using materials from experimentally infected cultured cells, and applied in programs focused on pathogen discovery and the epidemiology of chronic diseases. Our initial focus will be two chronic diseases where specific viral taxa are associated and potentially implicated in pathogenesis: bronchioalveolar cell carcinoma and amyotrophic lateral sclerosis.
| Quan, Phenix-Lan; Firth, Cadhla; Conte, Juliette M et al. (2013) Bats are a major natural reservoir for hepaciviruses and pegiviruses. Proc Natl Acad Sci U S A 110:8194-9 |
| Allison, A B; Palacios, G; Travassos da Rosa, A et al. (2011) Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein. Virus Res 155:112-22 |
| Palacios, Gustavo; Wellehan Jr, James F X; Raverty, Stephen et al. (2011) Discovery of an orthoreovirus in the aborted fetus of a Steller sea lion (Eumetopias jubatus). J Gen Virol 92:2558-65 |
| Wünschmann, Arno; Honkavuori, Kirsi; Briese, Thomas et al. (2011) Antigen tissue distribution of Avian bornavirus (ABV) in psittacine birds with natural spontaneous proventricular dilatation disease and ABV genotype 1 infection. J Vet Diagn Invest 23:716-26 |
| Honkavuori, Kirsi S; Shivaprasad, H L; Briese, Thomas et al. (2011) Novel picornavirus in Turkey poults with hepatitis, California, USA. Emerg Infect Dis 17:480-7 |
| Quan, Phenix-Lan; Junglen, Sandra; Tashmukhamedova, Alla et al. (2010) Moussa virus: a new member of the Rhabdoviridae family isolated from Culex decens mosquitoes in Côte d'Ivoire. Virus Res 147:17-24 |
| Palacio, Gustavo; Briese, Thomas; Lipkin, W Ian (2010) Microbe hunting in laboratory animal research. ILAR J 51:245-54 |
| Lipkin, W Ian (2010) Microbe hunting. Microbiol Mol Biol Rev 74:363-77 |
| Baumeister, Elsa; Palacios, Gustavo; Cisterna, Daniel et al. (2010) Molecular characterization of severe and mild cases of influenza A (H1N1) 2009 strain from Argentina. Medicina (B Aires) 70:518-23 |
| Palacios, Gustavo; Savji, Nazir; Hui, Jeffrey et al. (2010) Genomic and phylogenetic characterization of Merino Walk virus, a novel arenavirus isolated in South Africa. J Gen Virol 91:1315-24 |
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