Persistent colonization of the human stomach with the Gram negative bacterium Helicobacter pylori is a risk factor for the development of gastric cancer and peptic ulcer disease. The long-term goals of this work are to understand the molecular mechanisms by which H. pylori causes these diseases, and to develop effective means for the prevention and treatment of these diseases. H. pylori isolates from different humans exhibit a considerable level of genetic diversity. One chromosomal region that is present in some H. pylori strains but not others is the cag pathogenicity island (PAI), a 40 kb region that is predicted to encode 27 different proteins. The incidence of symptomatic gastroduodenal disease (gastric cancer or peptic ulceration) is higher among persons infected with cag PAI-positive strains than among persons infected with cag-negative strains. The cag PAI encodes an effector protein, CagA, and multiple proteins that translocate CagA into gastric epithelial cells via a type IV secretion process. This project describes plans to investigate the molecular mechanisms by which CagA is translocated into gastric epithelial cells.
The aims of the project are i) to analyze subassemblies of the cag PAI-encoded type IV secretion apparatus;ii) to investigate the subcellular localization of H. pylori Cag proteins;and iii) to analyze effects of CagA in the stomachs of animal models. These studies should lead to important advances in our understanding of the molecular mechanisms by which products of the H. pylori cag PAI contribute to the development of gastroduodenal diseases. The presence of a bacterium known as Helicobacter pylori in the human stomach contributes to the development of cancer of the stomach and peptic ulcer disease. This research seeks to understand how a bacterial infection can lead to these diseases. The long-term goals are to develop effective means for the prevention and treatment of stomach cancer and peptic ulcer disease.
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| Voss, Bradley J; Gaddy, Jennifer A; McDonald, W Hayes et al. (2014) Analysis of surface-exposed outer membrane proteins in Helicobacter pylori. J Bacteriol 196:2455-71 |
| Kodaman, Nuri; Pazos, Alvaro; Schneider, Barbara G et al. (2014) Human and Helicobacter pylori coevolution shapes the risk of gastric disease. Proc Natl Acad Sci U S A 111:1455-60 |
| Johnson, Elizabeth M; Gaddy, Jennifer A; Voss, Bradley J et al. (2014) Genes required for assembly of pili associated with the Helicobacter pylori cag type IV secretion system. Infect Immun 82:3457-70 |
| Gaddy, Jennifer A; Radin, Jana N; Loh, John T et al. (2014) The host protein calprotectin modulates the Helicobacter pylori cag type IV secretion system via zinc sequestration. PLoS Pathog 10:e1004450 |
| McClain, Mark S; Duncan, Stacy S; Gaddy, Jennifer A et al. (2013) Control of gene expression in Helicobacter pylori using the Tet repressor. J Microbiol Methods 95:336-41 |
| Cover, Timothy L; Peek Jr, Richard M (2013) Diet, microbial virulence, and Helicobacter pylori-induced gastric cancer. Gut Microbes 4:482-93 |
| Gaddy, Jennifer A; Radin, Jana N; Loh, John T et al. (2013) High dietary salt intake exacerbates Helicobacter pylori-induced gastric carcinogenesis. Infect Immun 81:2258-67 |
| Radin, Jana N; Gaddy, Jennifer A; González-Rivera, Christian et al. (2013) Flagellar localization of a Helicobacter pylori autotransporter protein. MBio 4:e00613-12 |
| Serrano, Carolina; Wright, Shelton W; Bimczok, Diane et al. (2013) Downregulated Th17 responses are associated with reduced gastritis in Helicobacter pylori-infected children. Mucosal Immunol 6:950-959 |
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