Neisseria meningitidis is responsible for sepsis and meningitis worldwide. Currently no vaccine against serogroup B strains is available in the U.S. but a serogroup B vaccine recently was licensed in the European Union, Australia and Canada. This vaccine contains factor H binding protein (fHbp) as one of its principal antigens. The protein recruits complement factor H (fH) to the bacterial surface, which allows the bacteria to evade host immunity. Binding of fH to fHbp is specific for human fH and, as we recently discovered, for fH in a subset of rhesus macaques. Based on studies in transgenic mice that expressed human fH, binding of fH to the fHbp vaccine decreased protective antibody responses. Further, mutant fHbp vaccines with single amino acid substitutions that eliminated fH binding had increased protective antibody responses, compared to control fHbp vaccines that bound human fH. However, there can be differences in antibody repertoire between rodents and primates and there are many examples of promising vaccine candidates in mice that failed to elicit protection in humans. Therefore, immunogenicity studies in a nonhuman primate model where fH binds to the control fHbp vaccine, but not to the mutant vaccine, would provide compelling rationale for development of mutant fHbp vaccines for humans.
In Aim 1, we will compare protective serum antibody responses of rhesus macaques immunized with a recombinant mutant fHbp vaccine that does not bind rhesus fH to those of a control fHbp vaccine that binds rhesus fH.
In Aim 2, we will characterize the antibody repertoires to the two vaccines by generating recombinant anti-fHbp fragments (Fabs) from variable region genes isolated from B cells of vaccinated animals. The locations of the epitopes recognized by the Fabs will be defined using a mutant fHbp library in yeast. The Fabs also will be tested for inhibition of binding of fH to fHbp, which is important for anti-fHbp antibody protective activity. However, since Fabs do not engage C1q or activate complement, in Aim 3, we will investigate anti-fHbp antibody protective activity in relation to epitope location by constructing chimeric IgG antibodies containing combining sites from rhesus-derived antibodies and effector portions of human IgG1 antibodies. Using human complement, the chimeric antibodies will be tested for activation of C3b deposition on the bacterial surface and bactericidal activity, and passive protection against bacteremia in human fH transgenic infant rats. Collectively, the data will provide insight into mechanisms responsible for increased immunogenicity of a prototype fHbp vaccine engineered to have decreased fH binding. The results will be directly applicable to development of more effective meningococcal serogroup B vaccines for humans. The mutant vaccines also may be safer, because of less risk of inducing auto-reactive anti-fH antibodies.

Public Health Relevance

No vaccine is available in the U.S. for prevention of meningitis or sepsis caused by serogroup B strains of the bacterium Neisseria meningitidis. A promising vaccine candidate, factor H binding protein (fHbp), is known to bind to a host protein, called factor H (fH). Our study will determine whether a mutant fHbp vaccine engineered not to bind to fH elicits greater protective antibodies in immunized rhesus monkeys than a fHbp vaccine that binds fH. Because results from rhesus monkeys are likely to be relevant to human responses to vaccination, our studies have a high likelihood of leading to a more effective vaccine and provide a framework for future development of microbial vaccines based on antigens that bind to host proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI114701-03
Application #
9177750
Study Section
Vaccines Against Microbial Diseases Study Section (VMD)
Program Officer
GU, Xin-Xing
Project Start
2014-12-01
Project End
2019-11-30
Budget Start
2016-12-01
Budget End
2017-11-30
Support Year
3
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Children's Hospital & Res Ctr at Oakland
Department
Type
DUNS #
076536184
City
Oakland
State
CA
Country
United States
Zip Code
94609
López-Sagaseta, Jacinto; Beernink, Peter T; Bianchi, Federica et al. (2018) Crystal structure reveals vaccine elicited bactericidal human antibody targeting a conserved epitope on meningococcal fHbp. Nat Commun 9:528
Nolfi-Donegan, Deirdre; Konar, Monica; Vianzon, Vianca et al. (2018) Fatal Nongroupable Neisseria meningitidis Disease in Vaccinated Patient Receiving Eculizumab. Emerg Infect Dis 24:
Konar, Monica; Granoff, Dan M (2017) Eculizumab treatment and impaired opsonophagocytic killing of meningococci by whole blood from immunized adults. Blood 130:891-899
Lujan, Eduardo; Partridge, Elizabeth; Giuntini, Serena et al. (2017) Breadth and Duration of Meningococcal Serum Bactericidal Activity in Health Care Workers and Microbiologists Immunized with the MenB-FHbp Vaccine. Clin Vaccine Immunol 24:
Giuntini, Serena; Lujan, Eduardo; Gibani, Malick M et al. (2017) Serum Bactericidal Antibody Responses of Adults Immunized with the MenB-4C Vaccine against Genetically Diverse Serogroup B Meningococci. Clin Vaccine Immunol 24:
Partridge, Elizabeth; Lujan, Eduardo; Giuntini, Serena et al. (2017) The role of anti-NHba antibody in bactericidal activity elicited by the meningococcal serogroup B vaccine, MenB-4C. Vaccine 35:4236-4244
Lujan, Eduardo; Pajon, Rolando; Granoff, Dan M (2016) Impaired Immunogenicity of Meningococcal Neisserial Surface Protein A in Human Complement Factor H Transgenic Mice. Infect Immun 84:452-8
Giuntini, S; Granoff, D M; Beernink, P T et al. (2016) Human IgG1, IgG3, and IgG3 Hinge-Truncated Mutants Show Different Protection Capabilities against Meningococci Depending on the Target Antigen and Epitope Specificity. Clin Vaccine Immunol 23:698-706
Granoff, Dan M; Giuntini, Serena; Gowans, Flor A et al. (2016) Enhanced protective antibody to a mutant meningococcal factor H-binding protein with low-factor H binding. JCI Insight 1:e88907
Pajon, Rolando; Lujan, Eduardo; Granoff, Dan M (2016) A meningococcal NOMV-FHbp vaccine for Africa elicits broader serum bactericidal antibody responses against serogroup B and non-B strains than a licensed serogroup B vaccine. Vaccine 34:643-649

Showing the most recent 10 out of 15 publications