Abstract

Work on the isolation and characterization of proglucagon, its cleavage intermediates and glucagon from anglerfish pancreatic islets is being continued. It is expected that the sequence analysis of glucagon will be completed during the budget year. Studies on the proteolytic processing of cell-free translation products and 10K - 17K dalton products from intact tissue will be continued as well. Subcellular conversion of both exogenous and endogenous labeled precursors are monitored in the presence or absence of various inhibitors of proteolysis. In collaborative studies, we will investigate the existence of detectable pre-prohormones in intact tissue and the generation of product peptides during cell-free translation of islet mRNA in the presence of dog pancreas microsomes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016921-11
Application #
3151034
Study Section
Metabolism Study Section (MET)
Project Start
1977-12-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Emory University
Department
Type
Schools of Medicine
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

Noe, B D; Amherdt, M; Perrelet, A et al. (1988) Simultaneous assessment of prohormone transport and processing in four separate islet cell types: a combined autoradiographic and biochemical study. Pancreas 3:700-13
McDonald, J K; Greiner, F; Wood, J G et al. (1987) Oxytocin-like immunoreactive nerves are associated with insulin-containing cells in pancreatic islets of anglerfish (Lophius americanus). Cell Tissue Res 249:7-12
Mackin, R B; Noe, B D (1987) Characterization of an islet carboxypeptidase B involved in prohormone processing. Endocrinology 120:457-68
McDonald, J K; Dees, W L; Ahmed, C E et al. (1987) Biochemical and immunocytochemical characterization of neuropeptide Y in the immature rat ovary. Endocrinology 120:1703-10
Noe, B D; Andrews, P C; Dixon, J E et al. (1986) Cotranslational and posttranslational proteolytic processing of preprosomatostatin-I in intact islet tissue. J Cell Biol 103:1205-11
Noe, B D; Andrews, P C (1986) Specific glucagon-related peptides isolated from anglerfish islets are metabolic cleavage products of (pre)proglucagon-II. Peptides 7:331-6
Mackin, R B; Jones, D P; Noe, B D (1986) Islet secretory granules contain cytochrome b561. Diabetes 35:881-5
Noe, B D; McDonald, J K; Greiner, F et al. (1986) Anglerfish islets contain NPY immunoreactive nerves and produce the NPY analog aPY. Peptides 7:147-54
Noe, B D; Spiess, J (1985) Post-translational processing of anglerfish islet somatostatin precursors. Adv Exp Med Biol 188:123-40
Spiess, J; Noe, B D (1985) Processing of an anglerfish somatostatin precursor to a hydroxylysine-containing somatostatin 28. Proc Natl Acad Sci U S A 82:277-81

Showing the most recent 10 out of 12 publications