Long-Term Objectives: Determination of the programmed and environmental controls operating in the differentiation of normal skeletal muscle cells. Applicability of regeneration, aging.
Specific Aims : Testing of predictions and general validity of a lineage model of myogenesis both in vitro and in vivo. In particular: 1. Isolation and characterization of the myogenic stem cell. a. Determination of which proteins or active genes are unique to this cell. b. Determination of the environmental controls that affect the types of progeny produced by this cell. c. Determination of whether this cell decides what types of proteins are to be synthesized at the end of the lineage. d. Determination of the relationship of this cell to muscle fibroblasts. 2. Testing the lineage model in vivo in the chick embryo. 3. Testing the model in other species. 4. Identification of the lineage stage-specific markets (genetic and/or protein) and markers for non-myogenic cells. 5. Determination of chromatin structural changes as cells traverse the lineage. Methodology: Tissue culture, protein purification, polyclonal and monlclonal antibody production and characterization, autoradiography, cell fractionation by density gradient centrifugation, cloning of chick embryo precursor cell genes (cDNA's), immunocytochemistry.
| Nameroff, M; Rhodes, L D (1989) Differential response among cells in the chick embryo myogenic lineage to photosensitization by Merocyanine 540. J Cell Physiol 141:475-82 |
| Yablonka-Reuveni, Z (1989) The emergence of the endothelial cell lineage in the chick embryo can be detected by uptake of acetylated low density lipoprotein and the presence of a von Willebrand-like factor. Dev Biol 132:230-40 |
| Yablonka-Reuveni, Z (1988) Discrimination of myogenic and nonmyogenic cells from embryonic skeletal muscle by 90 degrees light scattering. Cytometry 9:121-5 |
