Core - Molecular Biology

Abstract

The Molecular Biology Core will support the various projects in this program by providing the following reagents and services: 1. Isolation and maintenance of shared pools of characterized osteoblast and osteosarcoma RNAs; northern blot analysis and nuclear run-on analysis as necessary. 2. Oligonucleotide synthesis. 3. Preparation and labeling of probes used routinely or in common by the projects. 4. Growth of recombinant DNA plasmids and phage. 5. Maintenance and permanent storage of plasmid, bacterial, and phage stocks (including bacterial hosts, vectors, libraries, etc.). 6. Preparation of plates and media for bacterial culture. 7. Construction of cDNA libraries. 8. Assistance with special projects, such as screening of cDNA and genomic libraries, or large DNA sequencing projects.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Project (R01)
• Project #: 5R01AR045688-01
• Application #: 2777893
• Budget Start: 1997-10-01
• Budget End: 1998-09-30
• Support Year: 1
• Fiscal Year: 1998

Institution

• Name: University of Massachusetts Medical School Worcester
• DUNS #: 660735098
• City: Worcester
• State: MA
• Country: United States
• Zip Code: 01655

Publications

Ali, Syed A; Zaidi, Sayyed K; Dobson, Jason R et al. (2010) Transcriptional corepressor TLE1 functions with Runx2 in epigenetic repression of ribosomal RNA genes. Proc Natl Acad Sci U S A 107:4165-9

Stein, G S; Stein, J L; Lian, J B et al. (2004) Nuclear microenvironments: an architectural platform for the convergence and integration of transcriptional regulatory signals. Eur J Histochem 48:65-76

Stein, Gary S; Lian, Jane B; van Wijnen, Andre J et al. (2004) Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression. Oncogene 23:4315-29

Stein, Gary S; Lian, Jane B; Montecino, Martin et al. (2003) Nuclear microenvironments support physiological control of gene expression. Chromosome Res 11:527-36

Lian, Jane B; Stein, Janet L; Stein, Gary S et al. (2003) Runx2/Cbfa1 functions: diverse regulation of gene transcription by chromatin remodeling and co-regulatory protein interactions. Connect Tissue Res 44 Suppl 1:141-8

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Temporal and spatial parameters of skeletal gene expression: targeting RUNX factors and their coregulatory proteins to subnuclear domains. Connect Tissue Res 44 Suppl 1:149-53

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer. Blood Cells Mol Dis 30:170-6

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression. J Cell Biochem 88:340-55

Drissi, Hicham; Pouliot, Arlyssa; Koolloos, Christian et al. (2002) 1,25-(OH)2-vitamin D3 suppresses the bone-related Runx2/Cbfa1 gene promoter. Exp Cell Res 274:323-33

Zaidi, Sayyed K; Sullivan, Andrew J; van Wijnen, Andre J et al. (2002) Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites. Proc Natl Acad Sci U S A 99:8048-53