Abstract

The aim of this proposal is to prepare monoclonal antibodies against the human breast cancer cell protein 24k. The antibodies will be prepared by the methodology of Kohler and Milstein and be used to establish a radioimmunoassay for 24k in breast cancer biopsies. A study will be done to compare estrogen and progesterone receptor with 24k in predicting responsiveness to endocrine therapy of breast cancer. The antibodies will also be used to select subsets of breast cancer tissue culture cells particularly responsive to estradiol.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA011378-18
Application #
3163465
Study Section
Pathology B Study Section (PTHB)
Project Start
1977-12-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
18
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Fuqua, S A; Oesterreich, S; Hilsenbeck, S G et al. (1994) Heat shock proteins and drug resistance. Breast Cancer Res Treat 32:67-71
Fuqua, S A; Benedix, M G; Krieg, S et al. (1994) Constitutive overexpression of the 27,000 dalton heat shock protein in late passage human breast cancer cells. Breast Cancer Res Treat 32:177-86
Ciocca, D R; Oesterreich, S; Chamness, G C et al. (1993) Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review. J Natl Cancer Inst 85:1558-70
Oesterreich, S; Weng, C N; Qiu, M et al. (1993) The small heat shock protein hsp27 is correlated with growth and drug resistance in human breast cancer cell lines. Cancer Res 53:4443-8
Damstrup, L; Andersen, J; Kufe, D W et al. (1992) Immunocytochemical determination of the estrogen-regulated proteins Mr 24,000, Mr 52,000 and DF3 breast cancer associated antigen: clinical value in advanced breast cancer and correlation with estrogen receptor. Ann Oncol 3:71-7
McGuire, W L; Hilsenbeck, S; Clark, G M (1992) Optimal mastectomy timing. J Natl Cancer Inst 84:346-8
Ciocca, D R; Fuqua, S A; Lock-Lim, S et al. (1992) Response of human breast cancer cells to heat shock and chemotherapeutic drugs. Cancer Res 52:3648-54
McGuire, W L; Tandon, A K; Allred, D C et al. (1992) Treatment decisions in axillary node-negative breast cancer patients. J Natl Cancer Inst Monogr :173-80
Sunderland, M C; McGuire, W L (1990) Prognostic indicators in invasive breast cancer. Surg Clin North Am 70:989-1004
Andersen, J; Skovbon, H; Poulsen, H S (1989) Immunocytochemical determination of the estrogen-regulated protein Mr 24,000 in primary breast cancer and response to endocrine therapy. Eur J Cancer Clin Oncol 25:641-3

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