Abstract

The most important objective in this research is to study the metabolism of N?G?-monomethyl-L-arginine, an endogenously synthesized compound, and to identify one of the metabolites as N-methylurea, a compound which can be nitrosated to give rise to 1-N-methyl,1-N-nitrosourea, one of the most potent carcinogens known. This will be pursued with the following aims: (1) to study the metabolic pathways of N?G?-monomethyl-L-arginine which involve hydrolytic cleavage, oxidative demethylation, and decarboxylation; (2) to investigate the possible formation of N-methylurea from N?G?-monomethyl-L-arginine; (3) to further purify protein methylase I which methylates the guanadine group of protein-bound arginine residues; and (4) to investigate the possible antitumor effects of N?G?-monomethyl-L-arginine on tumor cells in tissue or cell culture. (K)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA012226-14
Application #
3163620
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1976-06-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122

  Publications

Schwartz, Justin; Holmuhamedov, Ekhson; Zhang, Xun et al. (2013) Minocycline and doxycycline, but not other tetracycline-derived compounds, protect liver cells from chemical hypoxia and ischemia/reperfusion injury by inhibition of the mitochondrial calcium uniporter. Toxicol Appl Pharmacol 273:172-9
Park, I K; Shin, S; Park, K S et al. (1988) Interrelationship between nuclear histone binding and cell proliferation. Int J Biochem 20:183-7
Park, K S; Kim, S; Paik, W K (1987) Histone binding to isolated rat liver nuclei. Int J Biochem 19:455-9
Cho, Y B; Park, K S; Kim, S et al. (1987) Synthesis of NG-monomethylagmatine, a decarboxylation product of NG-monomethyl-L-arginine. Anal Biochem 160:429-33
Trezl, L; Park, K S; Kim, S et al. (1987) Studies on in vitro S-methylation of naturally occurring thiol compounds with N-methyl-N-nitrosourea and methyl methanesulfonate. Environ Res 43:417-26
Paik, W K; Kim, S (1986) Enzymology of protein methylation. Yonsei Med J 27:159-77
Disa, S G; Gupta, A; Kim, S et al. (1986) Site specificity of histone H4 methylation by wheat germ protein-arginine N-methyltransferase. Biochemistry 25:2443-8
Suh, B; Lee, H W; Hong, S Y et al. (1986) A new HPLC analytical method for o-hydroxyhippuric acid in uremic serum. J Biochem Biophys Methods 13:211-20
Kim, S; Tuck, M; Ho, L L et al. (1986) Myelin basic protein-specific protein methylase I activity in shiverer mutant mouse brain. J Neurosci Res 16:357-65
Jun, G J; Ro, J Y; Kim, M H et al. (1986) Studies on the distribution of O6-methylguanine-DNA methyltransferase in rat. Biochem Pharmacol 35:377-84

Showing the most recent 10 out of 13 publications