Prostatic nuclear cAMP-independent protein kinases (PKs) active towards non-histone proteins (including nuclear matrix proteins) have been established by us to demonstrate early androgenic modulation which may be critical to the initial events in the androgenic control of gene expression in the prostate. We have purified two such nuclear cAMP-independent PKs (N1 and N2). Both of the enzymes phosphorylated phosvitin and casein, but PK-N2 was more active towards non-histone proteins. Similar enzymes in other systems have been implicated in the control of activities related to gene action. We are currently raising antibodies against the two enzymes. For PK-N2, hybridomas generating monoclonal antibodies have been developed. The availability of specific antibodies should facilitate planned studies of cellular regulation and molecular biological control of these PKs in regard to their role in androgen-mediated growth control in the prostate. Relevant to this is our observation that purified prostatic androgen receptor is phosphorylated by one of the androgen-sensitive nuclear PKs. One of our goals has been to examine the nuclear proteins and protein kinases of human prostatic chromatin to identify features which might differ in the neoplastic prostate as compared with the normal. We have completed a study of the general properties of the human normal and benign hypertrophic prostate (BPH) chromatin-associated protein kinase reactions. There was no change in the activity of the BPH chromatin as compared with the normal prostate chromatin when tested with phosvitin or lysine-rich histone as substrate. However, a marked increase (by 150%) was found in PK, the rate of phosphorylation of endogenous chromatin-associated, non-histone proteins in BPH chromatin as compared with the normal. The underlying mechanism of this observation and also similar studies of the chromatin from prostatic carcinoma are in progress. (N)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA015062-12
Application #
3164080
Study Section
Experimental Therapeutics Subcommittee 2 (ET)
Project Start
1976-12-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Wang, Guixia; Pan, Yunqian; Ahmad, Kashif A et al. (2010) Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells. Biochemistry 49:3842-52
Hanif, Ismail M; Ahmad, Kashif A; Ahmed, Khalil et al. (2009) Involvement of reactive oxygen species in apoptosis induced by pharmacological inhibition of protein kinase CK2. Ann N Y Acad Sci 1171:591-9
Trembley, J H; Wang, G; Unger, G et al. (2009) Protein kinase CK2 in health and disease: CK2: a key player in cancer biology. Cell Mol Life Sci 66:1858-67
McDonnell, Maureen A; Abedin, Md Joynal; Melendez, Manuel et al. (2008) Phosphorylation of murine caspase-9 by the protein kinase casein kinase 2 regulates its cleavage by caspase-8. J Biol Chem 283:20149-58
Ahmad, Kashif A; Wang, Guixia; Unger, Gretchen et al. (2008) Protein kinase CK2--a key suppressor of apoptosis. Adv Enzyme Regul 48:179-87
Wang, Guixia; Ahmad, Kashif A; Harris, Nathan H et al. (2008) Impact of protein kinase CK2 on inhibitor of apoptosis proteins in prostate cancer cells. Mol Cell Biochem 316:91-7
Ahmad, Kashif A; Harris, Nathan H; Johnson, Andrew D et al. (2007) Protein kinase CK2 modulates apoptosis induced by resveratrol and epigallocatechin-3-gallate in prostate cancer cells. Mol Cancer Ther 6:1006-12
He, Hongbin; Tan, Mingjia; Pamarthy, Deepika et al. (2007) CK2 phosphorylation of SAG at Thr10 regulates SAG stability, but not its E3 ligase activity. Mol Cell Biochem 295:179-88
Wang, Guixia; Ahmad, Kashif A; Ahmed, Khalil (2006) Role of protein kinase CK2 in the regulation of tumor necrosis factor-related apoptosis inducing ligand-induced apoptosis in prostate cancer cells. Cancer Res 66:2242-9
Wang, Guixia; Ahmad, Kashif A; Unger, Gretchen et al. (2006) CK2 signaling in androgen-dependent and -independent prostate cancer. J Cell Biochem 99:382-91

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