We will continue our studies of the behavior of murine hematopoietic cells modified by acquired drug-resistance genes. We have recently cloned the gene for a mutant dihydrofolate reductase (DHFR) that is enzymatically active but highly resistant to inhibition by methotrexate (MTX) and certain other folic acid antagonists. We wish to test the effectiveness of the cloned gene in transforming mouse bone marrow cells to a state of MTX resistance. We plan to examine alternative strategies for modulating the transcription of the inserted drug-resistance gene sequences so that gene expression can be modified by the imposition of compounds such as glucocorticoids. We have developed a new technique for introducing macromolecules into mammalian cells and are exploring some of the variables associated with this methodology. (M)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA015619-13
Application #
3164231
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1977-06-01
Project End
1988-10-31
Budget Start
1987-07-01
Budget End
1988-10-31
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
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Zhou, D; Battifora, H; Yokota, J et al. (1987) Association of multiple copies of the c-erbB-2 oncogene with spread of breast cancer. Cancer Res 47:6123-5

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