During the last year we have determined the nucleotide sequence of: 1) the long terminal repeats of integrated M-MLV, integrated AKR, and unintegrated M-MLV and M-MSV, 2) the viral src gene and its cellular homologue, 3) the junction between viral and cellular src gene, 4) the portions of gag, pol, and env gene of M-MLV and M-MSV, and 5) we have used purified AMV reverse transcriptase for synthesis of double-stranded viral DNA from purified virions of M-MLV. Such double-stranded DNA molecules were molecularly cloned in plasmids. Five M-MLV specific plasmids were identified. Our future goals are to: 1) continue nucleotide sequence analysis of the viral gene, 2) use reverse transcriptase for creating site-directed mtagenesis in vitro, and 3) identify the pol gene product in the infected cell by raising antisera to peptide synthesized from nucleotide sequence analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA016561-12
Application #
3164430
Study Section
Experimental Virology Study Section (EVR)
Project Start
1977-09-01
Project End
1988-02-29
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037