We are using mouse embryonal carcinoma (EC) cell cultures to study the genetic and epigenetic controls on the transition from embryonic stem cells to differentiated cells. The H6 EC line can be induced to aggregate and then to compact by regulating the concentration of calcium in the growth medium and induced to differentiate by exposure to retinoic acid. These three processes are being examined, since they have features that parallel early developmental stages of the preimplantation mouse embryo and therefore could lead to a better understanding of normal and abnormal development. Stem cell aggregation is being studied by utilizing genetic variants in which the inability to aggregate is associated with altered membrane glycoprotein patterns. Compaction of the H6 aggregate is being characterized functionally and ultrastructurally. The controls over compaction and its significance to subsequent cell differentiation are being pursued. The retinoic-induced differentiation of H6 stem cells to endoderm-like cells is being studied by achieving rescue from the terminal state through cell hybridization or DNA transfection. (P)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA016754-13
Application #
3164499
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1974-12-01
Project End
1990-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
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