Abstract

We are using mouse embryonal carcinoma (EC) cell cultures to study the genetic and epigenetic controls on the transition from embryonic stem cells to differentiated cells. The H6 EC line can be induced to aggregate and then to compact by regulating the concentration of calcium in the growth medium and induced to differentiate by exposure to retinoic acid. These three processes are being examined, since they have features that parallel early developmental stages of the preimplantation mouse embryo and therefore could lead to a better understanding of normal and abnormal development. Stem cell aggregation is being studied by utilizing genetic variants in which the inability to aggregate is associated with altered membrane glycoprotein patterns. Compaction of the H6 aggregate is being characterized functionally and ultrastructurally. The controls over compaction and its significance to subsequent cell differentiation are being pursued. The retinoic-induced differentiation of H6 stem cells to endoderm-like cells is being studied by achieving rescue from the terminal state through cell hybridization or DNA transfection. (P)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA016754-13
Application #
3164499
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1974-12-01
Project End
1990-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Dahl, R E; Bernhisel-Broadbent, J; Scanlon-Holdford, S et al. (1995) Sleep disturbances in children with atopic dermatitis. Arch Pediatr Adolesc Med 149:856-60
James, J M; Bernhisel-Broadbent, J; Sampson, H A (1994) Respiratory reactions provoked by double-blind food challenges in children. Am J Respir Crit Care Med 149:59-64
Bernhisel-Broadbent, J; Dintzis, H M; Dintzis, R Z et al. (1994) Allergenicity and antigenicity of chicken egg ovomucoid (Gal d III) compared with ovalbumin (Gal d I) in children with egg allergy and in mice. J Allergy Clin Immunol 93:1047-59
Bernhisel-Broadbent, J; Strause, D; Sampson, H A (1992) Fish hypersensitivity. II: Clinical relevance of altered fish allergenicity caused by various preparation methods. J Allergy Clin Immunol 90:622-9
Sampson, H A; James, J M; Bernhisel-Broadbent, J (1992) Safety of an amino acid-derived infant formula in children allergic to cow milk. Pediatrics 90:463-5
Bernhisel-Broadbent, J; Scanlon, S M; Sampson, H A (1992) Fish hypersensitivity. I. In vitro and oral challenge results in fish-allergic patients. J Allergy Clin Immunol 89:730-7
Weaver, J D; Stetten, G; Littlefield, J W (1991) Partial trisomies in two spontaneously arising long-lived human keratinocyte lines. In Vitro Cell Dev Biol 27A:670-5
Weng, D E; Littlefield, J W (1991) Loss of expression of the uvomorulin gene in compaction-defective embryonal carcinoma cells. Somat Cell Mol Genet 17:617-9
Rosenberg, C; Stetten, G; Kearns, W G et al. (1991) Origin of chromosome rearrangements in two long-lived human keratinocyte lines. In Vitro Cell Dev Biol 27A:823-5
Littlefield, J W; Whitehouse, L L (1990) Absence of uvomorulin in a slowly compacting variant of H6 embryonal carcinoma cells. Somat Cell Mol Genet 16:191-4

Showing the most recent 10 out of 16 publications