Abstract

The specific objectives of this research grant are: (1) To biochemically transform LTK negative cells with EBV DNA; (2) to determine the complexity of EBV DNA in LTK positive transformants; (3) to analyze the structure, sequence arrangement and location of viral DNA in LTK positive transformants; (4) to analyze EBV RNA in the transformants; (5) to develop a lymphocyte system for TK transformation; (6) to apply similar techniques to the analysis of cells transformed with specific fragments of EBV DNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA017281-11
Application #
3164633
Study Section
Virology Study Section (VR)
Project Start
1978-06-01
Project End
1988-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Tanner, J; Weis, J; Fearon, D et al. (1987) Epstein-Barr virus gp350/220 binding to the B lymphocyte C3d receptor mediates adsorption, capping, and endocytosis. Cell 50:203-13
Liebowitz, D; Kopan, R; Fuchs, E et al. (1987) An Epstein-Barr virus transforming protein associates with vimentin in lymphocytes. Mol Cell Biol 7:2299-308
Wang, F; Petti, L; Braun, D et al. (1987) A bicistronic Epstein-Barr virus mRNA encodes two nuclear proteins in latently infected, growth-transformed lymphocytes. J Virol 61:945-54
Pearson, G R; Luka, J; Petti, L et al. (1987) Identification of an Epstein-Barr virus early gene encoding a second component of the restricted early antigen complex. Virology 160:151-61
Reiss, C S; Wang, D; Ghosh, D et al. (1987) Recognition of EBV plasma membrane protein expressed on murine cells after gene transfer. J Immunol 139:711-4
Gong, M; Ooka, T; Matsuo, T et al. (1987) Epstein-Barr virus glycoprotein homologous to herpes simplex virus gB. J Virol 61:499-508
Whang, Y; Silberklang, M; Morgan, A et al. (1987) Expression of the Epstein-Barr virus gp350/220 gene in rodent and primate cells. J Virol 61:1796-807
Wang, F; Gregory, C D; Rowe, M et al. (1987) Epstein-Barr virus nuclear antigen 2 specifically induces expression of the B-cell activation antigen CD23. Proc Natl Acad Sci U S A 84:3452-6
Sample, J; Hummel, M; Braun, D et al. (1986) Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site. Proc Natl Acad Sci U S A 83:5096-100
Hummel, M; Arsenakis, M; Marchini, A et al. (1986) Herpes simplex virus expressing Epstein-Barr virus nuclear antigen 1. Virology 148:337-48

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