Abstract

Considerable progress has occurred in the past few years towards understanding the critical variables that regulate T-cell growth. The T-cell growth factor, interleukin 2 (IL-2), has been identified as a single variably glycosylated polypeptide with Mr = 15,500, has been purified to homogeneity, and the gene encoding the peptide has been molecularly cloned. The mechanism of interaction of IL-2 with activated T cells has been found to be comprised of a classic, polypeptide hormone-receptor system. Monoclonal antibodies have been prepared to IL-2 and its receptor. Consequently, the cellular and molecular reagents are available for experiments directed toward understanding IL-2 receptor-mediated DNA duplication and mitosis. (HF)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA017643-10
Application #
3164763
Study Section
Immunobiology Study Section (IMB)
Project Start
1979-01-01
Project End
1986-03-31
Budget Start
1985-01-01
Budget End
1986-03-31
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Dartmouth College
Department
Type
Schools of Medicine
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code

  Publications

Beadling, C; Johnson, K W; Smith, K A (1993) Isolation of interleukin 2-induced immediate-early genes. Proc Natl Acad Sci U S A 90:2719-23
Landgraf, B E; Goldstein, B; Williams, D P et al. (1992) Recombinant interleukin-2 analogs. Dynamic probes for receptor structure. J Biol Chem 267:18511-9
Zmuidzinas, A; Mamon, H J; Roberts, T M et al. (1991) Interleukin-2-triggered Raf-1 expression, phosphorylation, and associated kinase activity increase through G1 and S in CD3-stimulated primary human T cells. Mol Cell Biol 11:2794-803
Landgraf, B E; Williams, D P; Murphy, J R et al. (1991) Conformational perturbation of interleukin-2: a strategy for the design of cytokine analogs. Proteins 9:207-16
Ehlers, S; Smith, K A (1991) Differentiation of T cell lymphokine gene expression: the in vitro acquisition of T cell memory. J Exp Med 173:25-36
Caligiuri, M A; Zmuidzinas, A; Manley, T J et al. (1990) Functional consequences of interleukin 2 receptor expression on resting human lymphocytes. Identification of a novel natural killer cell subset with high affinity receptors. J Exp Med 171:1509-26
Landgraf, B; Cohen, F E; Smith, K A et al. (1989) Structural significance of the C-terminal amphiphilic helix of interleukin-2. J Biol Chem 264:816-22
Smith, K A (1988) Interleukin-2: inception, impact, and implications. Science 240:1169-76
Smith, K A (1988) The interleukin 2 receptor. Adv Immunol 42:165-79
Wang, H M; Smith, K A (1987) The interleukin 2 receptor. Functional consequences of its bimolecular structure. J Exp Med 166:1055-69

Showing the most recent 10 out of 18 publications