Abstract

We have developed a rapid, simple, and quantitative filter-binding assay which can separate covalent DNA-protein complexes from protein-free DNA. We have used the assay to detect and quantitate the adenovirus DNA-terminal protein complex. We propose to study the role and synthesis of the terminal protein during infection. We develop a model for the action of terminal protein during initiation of adenovirus DNA replication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA017699-10
Application #
3164779
Study Section
Experimental Virology Study Section (EVR)
Project Start
1978-09-30
Project End
1986-06-30
Budget Start
1985-05-01
Budget End
1986-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Oregon State University
Department
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339

  Publications

Bennett, K L; Pearson, G D (1993) Sequence conversion during postreplicative adenovirus overlap recombination. Proc Natl Acad Sci U S A 90:1397-401
Hu, C H; Xu, F Y; Wang, K et al. (1992) Symmetrical adenovirus minichromosomes have hairpin replication intermediates. Gene 110:145-50
Wang, K; Xu, F Y; Ahern, K G et al. (1991) Inverted repeats direct repair of adenovirus minichromosome ends. Virology 183:44-51
Ahern, K G; Wang, K; Xu, F Y et al. (1991) Strands hybridize in postreplicative adenovirus overlap recombination. Proc Natl Acad Sci U S A 88:105-9
Reed, R L; Ahern, K G; Pearson, G D et al. (1988) Crosslinking of DNA by dehydroretronecine, a metabolite of pyrrolizidine alkaloids. Carcinogenesis 9:1355-61
Lee, J S; Brown, W E; Graham, J S et al. (1986) Molecular characterization and phylogenetic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species. Proc Natl Acad Sci U S A 83:7277-81
Chow, K C; Pearson, G D (1985) Adenovirus infection elevates levels of cellular topoisomerase I. Proc Natl Acad Sci U S A 82:2247-51
Irwin, D M; Ahern, K G; Pearson, G D et al. (1985) Characterization of the bovine prothrombin gene. Biochemistry 24:6854-61
Wang, K; Pearson, G D (1985) Adenovirus sequences required for replication in vivo. Nucleic Acids Res 13:5173-87
Kurath, G; Ahern, K G; Pearson, G D et al. (1985) Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping. J Virol 53:469-76