The overall objective of this research is to study the epigenetic factors that favor, modify, or inhibit the expression of the malignant phenotype of undifferentiated embryonic cells and stem cells of teratocarcinoma. To this end teratocarcinomas are produced from 7-day mouse embryos transplanted under the kidney of immunologically compatible recipients. It has been shown that the yield of malignant tumors depends on host-related and embryo-related factors that either promote or inhibit teratocarcinogenesis. Among the host-related factors, the immune factors play a significant role. The genome of the embryo and the maternally transmitted factors also influence the teratocarcinogenesis. The malignant stem cells of murine teratocarcinomas, like their putative precursors in the embryo, express on their surface a fucopentaose moiety that can be recognized with monoclonal antibodies. Upon differentiation of either embryonic or tumor cells, this surface marker disappears from the cell surface. Antibodies recognizing this stage-specific embryonic antigen react with many tissues in the adult mouse and even humans and are thus not restricted or uniquely specific for mouse embryonal carcinoma cells. Human embryonal carcinoma cells, on the other hand, display surface characteristics that make them distinct from the equivalent mouse tumor cells. (M)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA023097-08
Application #
3166032
Study Section
Pathology B Study Section (PTHB)
Project Start
1977-07-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Hahnemann University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19129
Krishnamurthy, Partha; Schwab, Matthias; Takenaka, Kazumasa et al. (2008) Transporter-mediated protection against thiopurine-induced hematopoietic toxicity. Cancer Res 68:4983-9
Leu, F J; Damjanov, I (1988) Protease treatment combined with immunohistochemistry reveals heterogeneity of normal and neoplastic basement membranes. J Histochem Cytochem 36:213-20
Wewer, U M; Tichy, D; Damjanov, A et al. (1987) Distinct antigenic characteristics of murine parietal yolk sac laminin. Dev Biol 121:397-407
Laury-Kleintop, L D; Damjanov, I; Alhadeff, J A (1987) Antibody-affinity purification of novel alpha-L-fucosidase from mouse liver. Biochem J 245:589-93
Leu, F J; Engvall, E; Damjanov, I (1986) Heterogeneity of basement membranes of the human genitourinary tract revealed by sequential immunofluorescence staining with monoclonal antibodies to laminin. J Histochem Cytochem 34:483-9
Damjanov, I; Damjanov, A; Damsky, C H (1986) Developmentally regulated expression of the cell-cell adhesion glycoprotein cell-CAM 120/80 in peri-implantation mouse embryos and extraembryonic membranes. Dev Biol 116:194-202
Yen, Y; Lee, M C; Salzmann, M et al. (1986) Lectin binding sites on human endocervix: a comparison with secretory and proliferative endometrium. Anat Rec 215:262-6
Damjanov, I; Mildner, B; Knowles, B B (1986) Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues. Lab Invest 55:588-92
Clark, R K; Tani, Y; Damjanov, I (1986) Suppression of nonspecific binding of avidin-biotin complex (ABC) to proteins electroblotted to nitrocellulose paper. J Histochem Cytochem 34:1509-12
Wewer, U M; Damjanov, A; Weiss, J et al. (1986) Mouse endometrial stromal cells produce basement-membrane components. Differentiation 32:49-58

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