Abstract

This research project will examine the structure of DNA polymerases from normal and cancer cells. Combined biochemical and immunological techniques will be used to explore the structures of these enzymes present inside the cells as well as the structures of purified enzymes.
The specific aims i nclude: (1) purification and examination of subunit structures of eukaryotic DNA polymerases; (2) preparation and characterization of immunological reagents to be used in structural studies on the enzymes; and (3) examination of the intracellular and nascent peptide structures of eukaryotic DNA polymerases. (I)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA023365-08
Application #
3166112
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1977-09-01
Project End
1986-12-31
Budget Start
1985-09-01
Budget End
1986-12-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
U.S. Uniformed Services University of Health Science
Department
Type
Schools of Medicine
DUNS #
City
Bethesda
State
MD
Country
United States
Zip Code
20814

  Publications

Cannizzaro, L A; Bollum, F J; Huebner, K et al. (1988) Chromosome sublocalization of a cDNA for human DNA polymerase-beta to 8p11----p12. Cytogenet Cell Genet 47:121-4
Holmes, A M; Cheriathundam, E; Bollum, F J et al. (1986) Immunological analysis of the polypeptide structure of calf thymus DNA polymerase-primase complex. J Biol Chem 261:11924-30
Holmes, A M; Cheriathundam, E; Bollum, F J et al. (1985) Initiation of DNA synthesis by the calf thymus DNA polymerase-primase complex. J Biol Chem 260:10840-6
Plevani, P; Foiani, M; Valsasnini, P et al. (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260:7102-7