Abstract

The main objective of this proposal is to establish the role of Shope fibroma virus (SFV) in the genesis of abnormal growth. For this purpose two strains of SFV that behave entirely different in cell cultures, one promoting the formation of """"""""foci"""""""" and the other inducing rapid cell lysis, will be investigated. Comparative studies include determination of host DNA synthesis, characterization of virus-induced and structural proteins and analysis of the viral DNA by restriction endonucleases. The properties of the cells in the """"""""foci"""""""" will be examined to determine whether they have been permanently modified. In addition, transformation of primary cell cultures with SFV will be attempted. For these studies the viral genome will be inactivated and/or the cells will be pretreated with tumor promoters and chemical carcinogens. Information sought from the proposed work can be of potential importance to explain the molecular basis of both viral and chemical carcinogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA029262-06
Application #
3168614
Study Section
Pathology B Study Section (PTHB)
Project Start
1981-02-01
Project End
1989-06-30
Budget Start
1987-07-01
Budget End
1989-06-30
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Stellrecht, K A; Sperber, K; Pogo, B G (1992) Stimulation of lymphokines in Jurkat cells persistently infected with vaccinia virus. J Virol 66:2046-50
Chang, P Y; Lai, A C; Pogo, B G (1992) Attenuated deletion mutant of vaccinia virus IHD-W recovered virulence by reinsertion of a terminal restriction fragment. Microb Pathog 13:49-59
Pogo, B G; Lai, A C; Joesten, M E et al. (1991) Changes in cell gene expression in human leukemic cells persistently infected with vaccinia virus. Virus Res 19:131-8
Lai, A C; Chu, Y (1991) A rapid method for screening vaccinia virus recombinants. Biotechniques 10:564-5
Lai, A C; Pogo, B G (1989) Characterization of vaccinia virus deletion mutants isolated from persistently infected Friend erythroleukemia cells. Virus Res 12:239-50
Obom, K M; Pogo, B G (1989) Tumorigenic conversion of NIH 3T3 cells by transfection with Shope fibroma virus DNA. Virus Genes 3:5-10
Lai, A C; Pogo, B G (1989) Attenuated deletion mutants of vaccinia virus lacking the vaccinia growth factor are defective in replication in vivo. Microb Pathog 6:219-26
Pogo, B G; Obom, K M; Haddad, J et al. (1989) Shope fibroma: a model system to study tumorigenesis by poxviruses. Ann N Y Acad Sci 567:222-33
Obom, K M; Pogo, B G (1988) Characterization of the transformation properties of Shope fibroma virus. Virus Res 9:33-48
Pogo, B G; Lai, A C; Holland, J G et al. (1988) Differences in the susceptibility of human blood cell lines to vaccinia virus. Intervirology 29:11-20

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