Abstract

The replication of the endogenous avian retrovirus in non-permissive chicken cells will be examined. Biological studies will examine the nature of the restriction in different genetic inbred lines, the mechanism by which the endogenous virus persists in non-permissive cells and the type of genetic event that leads to emergence of the variant. In order to characterize the restricted event, the biological activity of unintergrated intermediates will be examined. Further, the integration of the endogenous viurs in ramdom and clonal populations of non-permissive cells will be characterized using restriction endonuclease and Southern DNA transfer analysis. Both the endogenous virus and the variant to which it gives rise will be cloned by recombinant DNA techniques. Biochemical analysis will identify those sequences characteristic of the variant that function to provide replication in non-permissive cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA032295-03
Application #
3170268
Study Section
Virology Study Section (VR)
Project Start
1983-06-01
Project End
1986-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Pizer, E S; Baba, T W; Humphries, E H (1992) Activation of the c-myb locus is insufficient for the rapid induction of disseminated avian B-cell lymphoma. J Virol 66:512-23
Filardo, E J; Humphries, E H (1991) An avian retrovirus expressing chicken pp59c-myc possesses weak transforming activity distinct from v-myc that may be modulated by adjacent normal cell neighbors. J Virol 65:6621-9
Carlson, L M; McCormack, W T; Postema, C E et al. (1990) Templated insertions in the rearranged chicken IgL V gene segment arise by intrachromosomal gene conversion. Genes Dev 4:536-47
Buerstedde, J M; Reynaud, C A; Humphries, E H et al. (1990) Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J 9:921-7
Humphries, E H; Filardo, E J (1990) The transforming activity of PP59C-MYC is weaker than that of v-myc. Curr Top Microbiol Immunol 166:259-65
Kim, S; Humphries, E H; Tjoelker, L et al. (1990) Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol Cell Biol 10:3224-31
Pizer, E; Humphries, E H (1989) RAV-1 insertional mutagenesis: disruption of the c-myb locus and development of avian B-cell lymphomas. J Virol 63:1630-40
Humphries, E H; Barth, C F; Pizer, E (1988) The development of three distinct avian B cell lymphomas. Curr Top Microbiol Immunol 141:58-66
Humphries, E H; Baba, T W (1986) Restrictions that influence avian leukosis virus-induced lymphoid leukosis. Curr Top Microbiol Immunol 132:215-20
Baba, T W; Humphries, E H (1986) Selective integration of avian leukosis virus in different hematopoietic tissues. Virology 155:557-66

Showing the most recent 10 out of 12 publications