The decrease in aflatoxin B1 (AFB1) induced liver tumor incidence associated with low dietary protein will be studied. We will examine the hypothesis which states that the lower tumor yield caused by the lower intake of protein is primarily associated with events occuring during the post-initiation phase. The Fisher 344 male rat will be the animal model of choice and the measurement of Gamma-glutamyl transpeptidase positive (GGT+) foci of altered hepatocytes will be employed as the indicator of early preneoplasia.
The specific aims are 1) to evaluate the kinetic relationship between AFB1 dose, dietary protein level and time of emergence of GGT+ foci, 2) to compare the contribution of AFB1 dose with dietary protein level on the emergence of GGT+ foci, 3) to develop a single AFB1 dose protocol to better separate initiation and postinitiation events, 4) to evaluate possible mechanisms concerned with cell proliferation, immune surveillance and tumor cell aggressiveness, and 5) to test whether the same phenomena occur in a full-term tumor study. The significance of this research is supported by the suggestion that high protein intake is a cancer risk factor, which is a suggestion based on rather comprehensive animal and human data. The development of a good tumor model system to study this relationship is very much needed.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA034205-04
Application #
3171961
Study Section
Pathology B Study Section (PTHB)
Project Start
1984-03-01
Project End
1989-02-28
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
Schools of Nutrition
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Campbell, T C (2017) Nutritional Renaissance and Public Health Policy. J Nutr Biol 3:124-138
Campbell, T Colin (2017) Cancer Prevention and Treatment by Wholistic Nutrition. J Nat Sci 3:
Hu, J F; Cheng, Z; Chisari, F V et al. (1997) Repression of hepatitis B virus (HBV) transgene and HBV-induced liver injury by low protein diet. Oncogene 15:2795-801
Bell, R C; Golemboski, K A; Dietert, R R et al. (1994) Long-term intake of a low-casein diet is associated with higher relative NK cell cytotoxic activity in F344 rats. Nutr Cancer 22:151-62
Bell, R C; Lanou, A J; Frongillo Jr, E A et al. (1994) Accuracy and reliability of total body electrical conductivity (TOBEC) for determining body composition of rats in experimental studies. Physiol Behav 56:767-73
Youngman, L D; Campbell, T C (1992) Inhibition of aflatoxin B1-induced gamma-glutamyltranspeptidase positive (GGT+) hepatic preneoplastic foci and tumors by low protein diets: evidence that altered GGT+ foci indicate neoplastic potential. Carcinogenesis 13:1607-13
Youngman, L D; Campbell, T C (1992) The sustained development of preneoplastic lesions depends on high protein intake. Nutr Cancer 18:131-42
Youngman, L D; Campbell, T C (1992) Attenuation of preneoplastic lesion development by dietary protein intervention: apparent persistence and regression. Cancer Lett 66:165-74
Potischman, N; McCulloch, C E; Byers, T et al. (1991) Associations between breast cancer, plasma triglycerides, and cholesterol. Nutr Cancer 15:205-15
Horio, F; Youngman, L D; Bell, R C et al. (1991) Thermogenesis, low-protein diets, and decreased development of AFB1-induced preneoplastic foci in rat liver. Nutr Cancer 16:31-41

Showing the most recent 10 out of 18 publications