Abstract

The overall objective of the work is to elucidate the molecular mechanism of cell-cell and intracellular communications that are triggered by the specific binding of the immunoglobulin G to Fc-gamma receptors (Fc-gamma-R) present on the surface of B cells, macrophages, and other cell types.
The specific aims are to: (1) determine the nature of an initial biochemical signal transmitted by Fc-gamma-Rs on the surface of murine macrophages and macrophage-cell lines and (2) investigate the biochemical sequence of events following the initial signal transmitted by the Fc-Fc-gamma-R interaction. These goals will be approached by: (1) isolation and characterization of Fc-gamma-Rs, phospholipase A?2?, adenylate cyclases, and cAMP-dependent protein kinases and (2) studies of Fc-gamma-R-triggered cyclooxygenase and lipooxygenase pathways, cAMP synthesis, and activation of cAMP-dependent protein kinases at the cell level. The results obtained should contribute to the understanding of the biochemical basis for the cellular interactions that play important roles in the immune response in general. (CS)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA035977-03
Application #
3173488
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1984-06-01
Project End
1987-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Li, Y; Ito, N; Suzuki, T et al. (1995) Dexamethasone inhibits nitric oxide-mediated cytotoxicity via effects on both macrophages and target cells. Immunopharmacology 30:177-86
Fujihara, M; Connolly, N; Ito, N et al. (1994) Properties of protein kinase C isoforms (beta II, epsilon, and zeta) in a macrophage cell line (J774) and their roles in LPS-induced nitric oxide production. J Immunol 152:1898-906
Fujihara, M; Ito, N; Pace, J L et al. (1994) Role of endogenous interferon-beta in lipopolysaccharide-triggered activation of the inducible nitric-oxide synthase gene in a mouse macrophage cell line, J774. J Biol Chem 269:12773-8
Muroi, M; Muroi, Y; Suzuki, T (1994) The binding of immobilized IgG2a to Fc gamma 2a receptor activates NF-kappa B via reactive oxygen intermediates and tumor necrosis factor-alpha 1. J Biol Chem 269:30561-8
Fujihara, M; Muroi, M; Muroi, Y et al. (1993) Mechanism of lipopolysaccharide-triggered junB activation in a mouse macrophage-like cell line (J774). J Biol Chem 268:14898-905
Muroi, M; Suzuki, T (1993) Role of protein kinase A in LPS-induced activation of NF-kappa B proteins of a mouse macrophage-like cell line, J774. Cell Signal 5:289-98
Muroi, M; Muroi, Y; Yamamoto, K et al. (1993) Influence of 3' half-site sequence of NF-kappa B motifs on the binding of lipopolysaccharide-activatable macrophage NF-kappa B proteins. J Biol Chem 268:19534-9
Suzuki, T (1991) Signal transduction mechanisms through Fc gamma receptors on the mouse macrophage surface. FASEB J 5:187-93
Novotney, M; Chang, Z L; Uchiyama, H et al. (1991) Protein kinase C in tumoricidal activation of mouse macrophage cell lines. Biochemistry 30:5597-604
Yamada, A; Suzuki, T (1989) Fc gamma 2b receptor-mediated phagocytosis by a murine macrophage-like cell line (P388D1) and peritoneal resident macrophages. Up-regulation by the inhibitors of phospholipase A2 and cyclooxygenase. J Immunol 142:2457-63

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