The purpose of this proposed research is to analyze the morphological and functional nature of acinar cell basophilic foci (ABF), induced by the pancreatic carcinogen 4-hydroxyaminoquinoline-1-oxide (4-HAQO), and to study their relationship, if any, to acinar cell acidophilic foci (AAF) and acinar cell nodules (AN), precursor lesions of acinar cell carcinoma.
The specific aims of this proposal are: 1) to investigate the initiation and sequential development of ABF, AAA and AN following a single and multiple injection(s) of 4-HAQO in regenerating and non-regenerating pancreas; 2) to determine whether the development of the various types of nodules can be modulated by soybean trypsin inhibitor; 3) to evaluate functional differences between ABF, AAF and AN by analyzing their proliferative capacity, synthesis and localization of pancreatic enzymes as demonstrated by histo- and immunochemistry, and concanavalin A receptors on their plasma membranes; 4) to develop methods for isolation of cells from ABF, AAF and AN; and 5) to characterize the cells of ABF, AAF and AN biochemically, by measuring mixed function oxidase enzyme activity, synthesis and sulfation of secretory proteins, their response to secretogogues, and quantitation and characterizations of lectins and cholecystokinin receptors.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA036043-03
Application #
3173554
Study Section
Pathology B Study Section (PTHB)
Project Start
1985-01-01
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
School of Medicine & Dentistry
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
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Rao, M S; Subbarao, V; Yeldandi, A V et al. (1987) Pancreatic acinar cell regeneration following copper deficiency-induced pancreatic necrosis. Int J Pancreatol 2:71-85
Rao, M S; Subbarao, V; Scarpelli, D G (1987) Atypical acinar cell lesions of the pancreas in mice induced by 4-hydroxyaminoquinoline-1-oxide. Int J Pancreatol 2:1-10
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