DNA ploidy patterns in cell nuclei from cytologic and histologic samples will be used to provide tumor diagnostic and prognostic information. Our rapid, computer-controlled, video-based microphotometer system allows the recording of DNA-ploidy patterns within minutes, with statistically valid sample sizes, thus making this valuable method practical as a routine clinical laboratory test. It is intended to standardize the methodology for this purpose, to assemble and examine a clinical database to correlate ploidy patterns with clinical implications, to establish objective measures for ploidy pattern evaluation, and to establish the sensitivity for the detection of aneuploidy. An inexpensive, microprocessor-based microscope attachment and graphic display has been designed as a prototype for a practical pathology laboratory instrument. (3)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA037352-05
Application #
3175192
Study Section
(SSS)
Project Start
1984-03-01
Project End
1989-03-31
Budget Start
1988-03-01
Budget End
1989-03-31
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
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Schark, C; Fulton, N; Yashiro, T et al. (1992) The value of measurement of ras oncogenes and nuclear DNA analysis in the diagnosis of Hurthle cell tumors of the thyroid. World J Surg 16:745-51;discussion 752
Minimo, C; Bartels, P H; Kim, D H et al. (1991) Value of the cribriformity factor in grading prostatic carcinoma. Anal Quant Cytol Histol 13:411-7
Bibbo, M; Kim, D H; Pfeifer, T et al. (1991) Histometric features for the grading of prostatic carcinoma. Anal Quant Cytol Histol 13:61-8

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