The object of this project is to apply results of recent advances in molecular biology, specifically molecular cloning of cellular oncogenes, synthesis of oncogene related peptides, and in situ chromosome hybridization to the diagnosis, evaluation, and monitoring of human leukemias--specifically to chronic myelogenous leukemia (CML). A novel 8 Kb RNA hybridizing with the normal, cellular homologue of the transforming gene of Abelson murine leukemia virus (c-abl) has been detected in patients with CML and the t(9,22) translocation (Ph' chromosome). It is not present in CML without the t(9,22) or other leukemias with rare exceptions. We will analyze patients with CML and related myeloproliferative disorders (Ph' negative CML) for this novel c-abl RNA. The objective is to define more precisely the Ph' negative leukemia and to correlate c-abl expression with disease phase and response to therapy. These RNA studies are to be complemented with analysis of c-abl related patients and by in situ chromosome hybridization using a v-abl probe. These studies will allow a more precise (molecular) definition of CML and related human leukemias. (6)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA038569-02
Application #
3176619
Study Section
(SSS)
Project Start
1985-06-01
Project End
1988-04-30
Budget Start
1986-05-01
Budget End
1987-04-30
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Gale, R P; Butturini, A (1992) Leukemia: stem cells, preleukemia and cure. Leukemia 6 Suppl 1:80-5
Henon, P R; Butturini, A; Gale, R P (1991) Blood-derived haematopoietic cell transplants: blood to blood? Lancet 337:961-3
Butturini, A; Gale, R P (1991) Relationship between clonality and transformation in acute leukemia. Leuk Res 15:1-7
Sun, G X; Wormsley, S; Sparkes, R S et al. (1991) Where does transformation occur in acute leukemia? Leuk Res 15:1183-9
Gale, R P (1991) Will more intensive chemotherapy of leukemia increase cures? Leuk Res 15:185-7
Gale, R P; Winston, D (1991) Intravenous immunoglobulin in bone marrow transplantation. Cancer 68:1451-3
Butturini, A; Gale, R P (1991) Detecting minimal residual leukemia. Cancer Genet Cytogenet 52:19-26
Bernstein, R; Gale, R P (1990) Do chromosome abnormalities determine the type of acute leukemia that develops in CML? Leukemia 4:65-8
Butturini, A; Gale, R P (1990) New strategies for T cell depletion. Bone Marrow Transplant 6:225-7
Feig, S A; Dreazen, O; Simon, M et al. (1988) B cell acute lymphoblastic leukemia (ALL) in donor cells following bone marrow transplantation for T cell ALL. Bone Marrow Transplant 3:331-7

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