Preneoplastic steps such as metaplasia, hyperplasia, and atypia are considered pathological steps in the development human carcinoma. Multiphasic oncogene activation during carcinogenesis is shown to occur in malignant neoplasia. Oncogene activation is usually determined by morphological transformation of NIH3T3 cells. It is proposed to demonstrate the activation of oncogenes in tumors induced in human pancreas explants, to determine and compare the temporal relationship between the activation of the oncogene and morphological steps in pancreas carcinogenesis. It is further proposed to develop a system for detection of oncogene activation other than NIH3T3 cells. This model will be based on the morphological transformation of human pancreas cell lines in vitro and their tumorigenicity in """"""""athymic nude"""""""" mice in vivo.
| Maheshwari, K K; Parsa, I (1991) Amplification and activation of c-Ki-ras oncogene in cell line developed from human pancreas explants. Biochem Int 25:233-40 |
| Sung, K L; Sung, L A; Crimmins, M et al. (1988) Dynamic changes in viscoelastic properties in cytotoxic T-lymphocyte-mediated killing. J Cell Sci 91 ( Pt 2):179-89 |
| Parsa, I (1988) Loss of a Mr 78,000 marker in chemically induced transplantable carcinomas and primary carcinoma of human pancreas. Cancer Res 48:2265-72 |
| Parsa, I; Pour, P M; Cleary, C M (1988) Amplification of c-Ki-ras-2 oncogene sequences in human carcinoma of pancreas. Int J Pancreatol 3:45-51 |
| Madahar, C; Parsa, I (1987) Progression in a chemically induced transplantable human pancreas carcinoma. Int J Pancreatol 2:183-94 |
| Parsa, I (1987) Modulation of a membrane-associated marker in progenitor cells of human pancreas carcinoma. Carcinogenesis 8:1399-404 |
