Abstract

This research is an extension of previous work using polyoma mutant viruses and viral sequences with recombinant DNA probes to study the gene expression requirements of teratocarcinoma cells. Teratocarcinoma cells consist of a stem-cell embryonal carcinoma (EC), which can be grown as a cell line under cell culture conditions, and then depending upon the cell line used, can be induced to differentiate to other cell types. We are using the viral and recombinant DNA probes to examine what DNA sequences are necessary for expression of genes in these different cell types and to try to select for cellular sequences which might play a role in cell type-specific gene expression. This work makes use of various gene transfer techniques, manipulation of small restriction fragments containing viral enhancement sequences, oligonucleotide synthesis to reconstruct regulatory sequences, and competition transfection experiments to examine whether regulatory sequences are competing for cellular factors, which play a role in enhancing gene expression in teratocarcinoma cells. (G)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA039066-03
Application #
3177806
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1985-02-01
Project End
1990-01-31
Budget Start
1986-02-01
Budget End
1987-01-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Cash, D E; Bock, C B; Schughart, K et al. (1997) Retinoic acid receptor alpha function in vertebrate limb skeletogenesis: a modulator of chondrogenesis. J Cell Biol 136:445-57
Carey, F J; Linney, E A; Pedersen, R A (1995) Allocation of epiblast cells to germ layer derivatives during mouse gastrulation as studied with a retroviral vector. Dev Genet 17:29-37
Underhill, T M; Cash, D E; Linney, E (1994) Constitutively active retinoid receptors exhibit interfamily and intrafamily promoter specificity. Mol Endocrinol 8:274-85
Smith, B R; Johnson, G A; Groman, E V et al. (1994) Magnetic resonance microscopy of mouse embryos. Proc Natl Acad Sci U S A 91:3530-3
Colbert, M C; Linney, E; LaMantia, A S (1993) Local sources of retinoic acid coincide with retinoid-mediated transgene activity during embryonic development. Proc Natl Acad Sci U S A 90:6572-6
LaMantia, A S; Colbert, M C; Linney, E (1993) Retinoic acid induction and regional differentiation prefigure olfactory pathway formation in the mammalian forebrain. Neuron 10:1035-48
Taketo, M; Hoopes, C; Howard, T A et al. (1993) Mapping of the mouse Rar loci encoding retinoic acid receptors RAR alpha, RAR beta and RAR gamma. Jpn J Genet 68:175-84
Liu, Q; Linney, E (1993) The mouse retinoid-X receptor-gamma gene: genomic organization and evidence for functional isoforms. Mol Endocrinol 7:651-8
Linney, E (1992) Retinoic acid receptors: transcription factors modulating gene regulation, development, and differentiation. Curr Top Dev Biol 27:309-50
Balkan, W; Colbert, M; Bock, C et al. (1992) Transgenic indicator mice for studying activated retinoic acid receptors during development. Proc Natl Acad Sci U S A 89:3347-51

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