Our earlier work with chicken and mouse model systems indicate that the activation of the myb oncogene involves specific deletions in the coding regions of the proto-oncogene. Both amino and carboxyl-terminal deletions were observed in both systems. Such deletions lead to the synthesis of truncated myb protine by the activated forms when compared to the normal gene products. It is not clear at this point whether these deletions are essential for the oncogenic activation of the proto-oncogene or if enhanced expression of the normal myb gene alone is adequate for transformation by this gene. We propose to study the mechanism of activation of chicken and mouse c-myb genes by constructing retroviral expression vectors containing the entire c-myb genes or a c-myb gene with specific deletions in its 5' and/or 3' sequences. We also propose to isolate large amounts of the normal and truncated versions of this protein using bacterial and yeast expression vectors. Following the purification of the recombinant myb proteins, we propose to carry out experiments aimed at understanding the biochemical differences between normal and truncated myb proteins.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA044463-03
Application #
3187083
Study Section
Molecular Biology Study Section (MBY)
Project Start
1987-05-01
Project End
1991-04-30
Budget Start
1989-05-01
Budget End
1991-04-30
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104