Abstract

The objective of this research is to demonstrate that the Ia-like molecule is the target of a human lymphokine (CIL) which shows inhibitory activity on progenitor cells of the hemopoietic system and on Ia?+? cells of the immune response. This 85-kilodalton protein factor, which is present in the supernatant of a clone (MT1) of our human T-cell hybrid, will be purified and tested: (1) in sensitive animals to determine its physiological activity; (2) in vitro, against bone marrow cells from patients with Ia?+? leukemia; and (3) in nude mice to determine whether it has protective activity against the growth of human or mouse Ia?+? tumors. (HF)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA044977-02
Application #
3187889
Study Section
Experimental Immunology Study Section (EI)
Project Start
1986-07-01
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
Overall Medical
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Farber, D L; Giorda, R; Nettleton, M Y et al. (1993) Rat class III Fc gamma receptor isoforms differ in IgG subclass-binding specificity and fail to associate productively with rat CD3 zeta. J Immunol 150:4364-75
Giorda, R; Lampasona, V; Kocova, M et al. (1993) Non-radioisotopic typing of human leukocyte antigen class II genes on microplates. Biotechniques 15:918-25
Nimgaonkar, V L; Ganguli, R; Rudert, W A et al. (1993) A negative association of schizophrenia with an allele of the HLA DQB1 gene among African-Americans. Schizophr Res 8:199-209
Carcassi, C; Giorda, R; Trucco, M et al. (1992) A novel HLA-DR4 haplotype generated by a rare recombinational event between DRB1 and DQA1 loci. Immunogenetics 36:338-40
Giorda, R; Weisberg, E P; Ip, T K et al. (1992) Genomic structure and strain-specific expression of the natural killer cell receptor NKR-P1. J Immunol 149:1957-63
Giorda, R; Trucco, M (1991) Mouse NKR-P1. A family of genes selectively coexpressed in adherent lymphokine-activated killer cells. J Immunol 147:1701-8
Giorda, R; Chambers, W H; Dahl, C A et al. (1990) Isolation and characterization of a cDNA that encodes the core protein of the cytolytic granule proteoglycan in rat natural killer cells. Nat Immun Cell Growth Regul 9:91-102
Boehm, B O; Manfras, B J; Rosak, C et al. (1990) TcR-alpha and TcR-beta dialellic RFLPs in insulin-dependent (type I) Caucasian diabetic patients. Diabetes Res 15:63-7
Giorda, R; Rudert, W A; Vavassori, C et al. (1990) NKR-P1, a signal transduction molecule on natural killer cells. Science 249:1298-300
Conrad, S; Trucco, M; Bohm, B O (1990) HLA-DQA1 and HLA-DQB1 DNA typing in multiplex IDDM families by use of sequence-specific oligonucleotide probes and genomic sequencing. Biomed Biochim Acta 49:1219-22

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