Abstract

The long term objective of this research is to elucidate cellular and viral factors which determine the oncogenicity of BK virus. Sequences within the BK virus enhancer will be defined with respect to their importance for gene expression, DNA replication and transformation. Cellular factors which interact with these sequences will be identified, and the mechanism of gene control by the enhancer analyzed. We will determine whether there is a correlation between expression of the early region and development of pathology in transgenic mice. Using cells transformed in culture, and tumor cells obtained from transgenic mice, we will study the mechanisms by which the BK virus tumor antigens induce transformation. We will determine the cellular localization of the BK tumor antigens, and determine whether they associate with known cellular growth regulators at those sites. We will measure the extent of complex formation with p53 and other cellular proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
7R01CA045033-02
Application #
3188007
Study Section
(SRC)
Project Start
1989-02-01
Project End
1993-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Missouri-Columbia
Department
Type
Schools of Medicine
DUNS #
112205955
City
Columbia
State
MO
Country
United States
Zip Code
65211