Abstract

The overall objective of this research project is to investigate the repair of non-dimer DNA damage induced by solar UV wavelengths (290-320 nm) that are thought to be important in the induction of skin cancer by sunlight and determine the biological significance of these repair pathways through study of mutant cell lines deficient in the repair of non-dimer DNA damage. The ICR 2A frog cell line will be used in this project primarily because it is highly proficient in enzymatic photoreactivation. In this process the UV-induced pyrimidine dimers are selectively removed through the action of DNA photolyase and photoreactivating light, leaving all other photoproducts intact. Therefore, cells can be exposed to 290-320 nm radiation and the pyrimidine dimers selectively removed. This leaves a nearly pure population of non-dimer photoproducts and it is then possible to specifically investigate the repair and biological significance of these lesions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA045078-02
Application #
3188070
Study Section
Radiation Study Section (RAD)
Project Start
1986-09-01
Project End
1989-06-30
Budget Start
1987-12-01
Budget End
1989-06-30
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Brown University
Department
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912

  Publications

Rosenstein, B S; Weinstock, M A; Habib, R (1999) Transmittance spectra and theoretical sun protection factors for a series of sunscreen-containing sun care products. Photodermatol Photoimmunol Photomed 15:75-80
Zhang, X; Rosenstein, B S; Wang, Y et al. (1997) Induction of 8-oxo-7,8-dihydro-2'-deoxyguanosine by ultraviolet radiation in calf thymus DNA and HeLa cells. Photochem Photobiol 65:119-24
Rosenstein, B S; Subramanian, D; Muller, M T (1997) The involvement of topoisomerase I in the induction of DNA-protein crosslinks and DNA single-strand breaks in cells of ultraviolet-irradiated human and frog cell lines. Radiat Res 148:575-9
Rosenstein, B S; Vaslet, C A (1995) Molecular cloning of the human gene SUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation. Somat Cell Mol Genet 21:255-63
Rosenstein, B S; Vaslet, C A (1995) Molecular cloning of the human gene SUVCC2 associated with mutagenesis following the induction of non-dimer DNA damages by solar UV radiation. J Photochem Photobiol B 28:203-11
Rosenstein, B S; Vaslet, C A; Rosenstein, R B (1995) Molecular cloning of the human gene SUVCC1 associated with the repair of nondimer DNA damage induced by solar UV radiation. Photochem Photobiol 61:142-8
Rosenstein, B S; Rosenstein, R B; Zamansky, G B (1992) Repair of DNA damage induced in systemic lupus erythematosus skin fibroblasts by simulated sunlight. J Invest Dermatol 98:469-74
Rosenstein, B S; Rosenstein, R B (1992) Survival, excision repair and inhibition of DNA synthesis in normal human skin fibroblasts exposed to simulated sunlight. J Photochem Photobiol B 16:247-55
Rosenstein, B S; Mitchell, D L (1991) The repair of DNA damages induced in normal human skin fibroblasts exposed to simulated sunlight. Radiat Res 126:338-42
Rosenstein, B S (1989) DNA strand breakage in normal and solar ultraviolet-sensitive ICR 2A frog cell lines exposed to solar ultraviolet wavelengths. Environ Mol Mutagen 13:50-3

Showing the most recent 10 out of 14 publications