Previous studies show that consumption of soy milk containing significant quantities of the phytoestrogens, daidzein and genistein, by females for one month, effectively reduces levels of endogenous ovarian hormones and adrenal androgen, which are recognized risk factors for breast cancer. A soya diet containing genistein and daidzein will be given to women during specific segments of the menstrual cycle to evaluate the value of these natural compounds in reducing breast cancer risk through suppression of ovarian hormones. The first study will include 15-30 regularly cycling women who will be studied over 17 months as they ingest one dose of soya isoflavones (>200 mg) on separate months at seven specific periods during the menstrual cycle. Pharmacologic test cycles will alternate with control cycles. Blood and urine samples will be analyzed for isoflavones and ovarian hormones. The Second study will include males who consume repeatedly a soya diet preferentially enriched with one isoflavone within a two month period. This study will investigate factors which increase the bioavailability of genistein and will determine if soya phytoestrogens affect gonadotropin levels in addition to steroid hormones. The purpose of the studies is to determine the most efficient timing of phytoestrogen administration and the minimum effective dose for future studies of the effects of soya phytoestrogens on endogenous markers for cancer risk in women.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA056273-07
Application #
2894923
Study Section
Metabolic Pathology Study Section (MEP)
Program Officer
Seifried, Harold E
Project Start
1991-09-30
Project End
2001-04-30
Budget Start
1999-05-01
Budget End
2001-04-30
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Texas Medical Br Galveston
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
041367053
City
Galveston
State
TX
Country
United States
Zip Code
77555
Ramanujam, V-M S; Nayeem, Fatima; Anderson, Karl E et al. (2017) Riboflavin as an independent and accurate biomarker for adherence in a randomized double-blind and placebo-controlled clinical trial. Biomarkers 22:508-516
Ramanujam, V M Sadagopa; Anderson, Karl E; Grady, James J et al. (2011) Riboflavin as an oral tracer for monitoring compliance in clinical research. Open Biomark J 2011:1-7
Nayeem, Fatima; Nagamani, Manubai; Anderson, Karl E et al. (2009) Dietary beta-tocopherol and linoleic acid, serum insulin, and waist circumference predict circulating sex hormone-binding globulin in premenopausal women. J Nutr 139:1135-42
Huang, Yafei; Anderson, Karl E; Nagamani, Manubai et al. (2008) Dietary intake of lactose as a strong predictor for secretor status of nipple aspirate fluid in healthy premenopausal nonlactating women. Clin Cancer Res 14:1386-92
Huang, Yafei; Nagamani, Manubai; Anderson, Karl E et al. (2007) A strong association between body fat mass and protein profiles in nipple aspirate fluid of healthy premenopausal non-lactating women. Breast Cancer Res Treat 104:57-66
Lu, L J; Anderson, K E; Grady, J J et al. (2001) Effects of an isoflavone-free soy diet on ovarian hormones in premenopausal women. J Clin Endocrinol Metab 86:3045-52
Wan, X S; Lu, L J; Anderson, K E et al. (2000) Urinary excretion of Bowman-Birk inhibitor in humans after soy consumption as determined by a monoclonal antibody-based immunoassay. Cancer Epidemiol Biomarkers Prev 9:741-7
Lu, L J; Anderson, K E; Grady, J J et al. (2000) Decreased ovarian hormones during a soya diet: implications for breast cancer prevention. Cancer Res 60:4112-21
Cao, S; Hudnall, S D; Kohen, F et al. (2000) Measurement of estrogen receptors in intact cells by flow cytometry. Cytometry 41:109-14
Lu, L J; Cree, M; Josyula, S et al. (2000) Increased urinary excretion of 2-hydroxyestrone but not 16alpha-hydroxyestrone in premenopausal women during a soya diet containing isoflavones. Cancer Res 60:1299-305

Showing the most recent 10 out of 19 publications