Abstract

This is a proposal to study the regulation of the myeloid specific gp91-phox gene, which is induced during terminal myeloid differentiation, coincident with the transition of a proliferation progenitor cell to a terminally differentiated phagocyte. The proposal centers around 3 specific aims.
The first aim focuses on determining the role each cis-element and cognate DNA binding protein plays in the myeloid specific induction of gp91-phox expression in response to specific stimuli.
Specific Aim 2 includes efforts to obtain cDNA clones of novel transcription factors that bind tot the gp91-phox promoter. DNA binding factors will be cloned by ligand screening of expression libraries using DNA binding site probes, or by DNA affinity chromatography.
Specific Aim 3 focuses on the study of a putative cDNA clone of HAF-1, a novel DNA binding protein whose binding site in the gp91-phox promoter is mutated in chronic granulomatous disease patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA058947-10
Application #
6497738
Study Section
Hematology Subcommittee 2 (HEM)
Program Officer
Mccarthy, Susan A
Project Start
1993-03-01
Project End
2004-01-31
Budget Start
2002-02-01
Budget End
2004-01-31
Support Year
10
Fiscal Year
2002
Total Cost
$231,823
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Pediatrics
Type
Schools of Medicine
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202