Abstract

It is proposed to perform an in vitro and animal in vivo investigation of 188Re labeled to several antibodies by several direct and indirect methods in a manner similar to that just completed for 99mTc by the applicant. Stability of the label will be assessed in vitro by saline and serum incubations and by a novel cysteine challenge assay. Biodistribution studies in normal and in tumor-bearing mice will provide information on the in vivo fate of each suitable label. The applicant anticipates that this research will show definitively the superiority of one labeling method (e.g. direct vs. indirect) in mice and will, therefore, provide an objective approach towards the eventual investigation of these reagents for therapy of cancer patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA059785-02
Application #
2100399
Study Section
Experimental Therapeutics Subcommittee 1 (ET)
Project Start
1994-08-01
Project End
1997-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Qu, T; Wang, Y; Zhu, Z et al. (2001) Different chelators and different peptides together influence the in vitro and mouse in vivo properties of 99Tcm. Nucl Med Commun 22:203-15
Chang, F; Qu, T; Rusckowski, M et al. (1999) NHS-MAS3: a bifunctional chelator alternative to NHS-MAG3. Appl Radiat Isot 50:723-32
Fogarasi, M; Pullman, J; Winnard Jr, P et al. (1999) Pretargeting of bacterial endocarditis in rats with streptavidin and 111In-labeled biotin. J Nucl Med 40:484-90
Hnatowich, D J; Chang, F; Qu, T et al. (1999) Technetium-99m labeled peptides--an investigation of multiple HPLC peaks. Appl Radiat Isot 50:911-21
Hnatowich, D J; Qu, T; Chang, F et al. (1998) Labeling peptides with technetium-99m using a bifunctional chelator of a N-hydroxysuccinimide ester of mercaptoacetyltriglycine. J Nucl Med 39:56-64
Gano, L; Patricio, L; Marques, E et al. (1998) Human polyclonal immunoglobulin labelled with technetium-99m via NHS-MAG3: a comparison of radiochemical behavior and biological efficacy with other labelling methods. Nucl Med Biol 25:395-403
Chang, F; Rusckowski, M; Qu, T et al. (1997) Early results in the irrational design of new bifunctional chelators. Cancer 80:2347-53
Rusckowski, M; Qu, T; Chang, F et al. (1997) Technetium-99m labeled epidermal growth factor-tumor imaging in mice. J Pept Res 50:393-401
Rusckowski, M; Qu, T; Chang, F et al. (1997) Pretargeting using peptide nucleic acid. Cancer 80:2699-705
Winnard Jr, P; Chang, F; Rusckowski, M et al. (1997) Preparation and use of NHS-MAG3 for technetium-99m labeling of DNA. Nucl Med Biol 24:425-32

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