Studies of the Cell & Molecular Biology of Acute Leukemi

Preisler, Harvey D.

Abstract

This grant will address several aspects of the potential affects of bioactive agents on leukemia cells in vivo. Leukemia cells will be obtained before and after administration of these agents and the effects of these agents on the distribution of cell cycle times amongst the leukemia cells within individual patients will be explored. The effects of these agents on the expression of the protooncogenes fms, myb, IL1beta and myc will be studied as well. Initially, we will study the effects of retinoic acid/interferon and rhGM-CSF. The studies will be extended to other agents once these initial studies are completed. Parallel evaluation of the bioactive agents in vitro will be conducted so that predictive in vitro assays can be developed.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 1R01CA060086-01
• Application #: 3203868
• Study Section: Special Emphasis Panel (SRC)
• Project Start: 1992-08-01
• Project End: 1992-11-30
• Budget Start: 1992-08-01
• Budget End: 1992-11-30
• Support Year: 1
• Fiscal Year: 1992

Institution

• Name: University of Cincinnati
• Type: Schools of Medicine
• City: Cincinnati
• State: OH
• Country: United States
• Zip Code: 45221

Publications

Li, B; Yang, J; Andrews, C et al. (2000) Telomerase activity in preleukemia and acute myelogenous leukemia. Leuk Lymphoma 36:579-87

Mundle, S; Venugopal, P; Shetty, V et al. (1999) The relative extent and propensity of CD34+ vs. CD34- cells to undergo apoptosis in myelodysplastic marrows. Int J Hematol 69:152-9

Preisler, H D; Gao, X Z; Ming, T et al. (1999) Marrow cytokine transcripts and the secondary hematologic disorders. Leuk Lymphoma 35:297-302

Gao, X Z; Bi, S; Copra, H et al. (1998) Cytokine gene activity in AML cells in vivo in patients. Leuk Res 22:429-38

Preisler, H D; Bi, S; Venugopal, P et al. (1997) Cytokines, molecular biological abnormalities, and acute myelogenous leukemia. Leuk Res 21:299-312

Handa, H; Hegde, U P; Kotelnikov, V M et al. (1997) Bcl-2 and c-myc expression, cell cycle kinetics and apoptosis during the progression of chronic myelogenous leukemia from diagnosis to blastic phase. Leuk Res 21:479-89

Braun, D P; Preisler, H D (1997) Cytolytic activity of peripheral blood blast cells from patients with acute myeloid leukemia. Leuk Lymphoma 27:459-67

Raza, A; Alvi, S; Borok, R Z et al. (1997) Excessive proliferation matched by excessive apoptosis in myelodysplastic syndromes: the cause-effect relationship. Leuk Lymphoma 27:111-8

Raza, A; Alvi, S; Broady-Robinson, L et al. (1997) Cell cycle kinetic studies in 68 patients with myelodysplastic syndromes following intravenous iodo- and/or bromodeoxyuridine. Exp Hematol 25:530-5

Handa, H; Hegde, U P; Kotelnikov, V M et al. (1997) The effects of 13-cis retinoic acid and interferon-alpha in chronic myelogenous leukemia cells in vivo in patients. Leuk Res 21:1087-96